Nematode fatty acid desaturase-like sequences

• US 6,875,595 B2
• Filed: 09/13/2002
• Issued: 04/05/2005
• Est. Priority Date: 09/13/2001
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Divergence, Inc. (Bayer AG)
• Inventors: McLaird, Merry B., Kloek, Andrew P., Williams, Deryck J., Davila-Aponte, Jennifer A., Bradley, John D., Xu, Siqun, Frevert, Anita M.
• Primary Examiner(s): Manjunath, Rao
• Assistant Examiner(s): Pak, Yong D.
• Application Number:
  US10/243,468
• Publication Number:
  US 20030180802A1
• Time in Patent Office: 935 Days
• Field of Search: 435/189, 435/440, 435/4, 435/6, 435/183, 536/23.2, 536/23.1, 530/350
• US Class Current: 435/189
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 15/8285 for nematode resistance
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)
• CPC Class Codes: G01N 2333/4353 from nematodes
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Nucleic acid molecules from nematodes encoding fatty acid desaturase polypeptides are described. Fatty acid desaturase-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of fatty acid desaturase-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators of fatty acid desaturase-like polypeptides, as well as methods for antibody production.

Claims

1. A purified polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:

9, wherein the polypeptide has fatty acid desaturase activity.

2. The purified polypeptide of claim 1, wherein the amino acid sequence is at least 98% identical to the amino acid sequence of SEQ ID NO:

9.

3. A purified polypeptide comprising the amino acid sequence of SEQ ID NO:

9.