DNA polymerase III holoenzyme
First Claim
Patent Images
1. An isolated DNA molecule encoding a protein δ
- ′
subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention is directed toward the 5 previously unknown genes, for subunits δ, δ′, χ, θ, and ψ, of the DNA polymerase III holoenzyme, and toward a unique man-made enzyme containing 5, preferably 6, protein subunits which shows the same activity as the naturally occurring 10 protein subunit DNA polymerase III holoenzyme.
-
Citations
19 Claims
-
1. An isolated DNA molecule encoding a protein δ
- ′
subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (2, 3, 4, 5, 6)
- ′
-
7. An isolated protein δ
- ′
subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13, when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (8, 9)
- ′
-
10. An isolated protein δ
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (11, 12)
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
-
13. An isolated DNA molecule encoding a protein δ
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (14, 15, 16, 17)
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
-
18. An isolated DNA molecule consisting of SEQ ID NO. 11 or SEQ ID NO. 12.
-
19. An isolated protein consisting of the amino acid sequence encoded by SEQ ID NO. 11 or SEQ ID NO. 12.
Specification