DNA polymerase III holoenzyme
First Claim
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1. An isolated DNA molecule encoding a protein δ
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subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
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Abstract
The present invention is directed toward the 5 previously unknown genes, for subunits δ, δ′, χ, θ, and ψ, of the DNA polymerase III holoenzyme, and toward a unique man-made enzyme containing 5, preferably 6, protein subunits which shows the same activity as the naturally occurring 10 protein subunit DNA polymerase III holoenzyme.
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Citations
19 Claims
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1. An isolated DNA molecule encoding a protein δ
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subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An isolated protein δ
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subunit of polymerase III holoenzyme, wherein a polymerase III holoenzyme comprising the subunit is capable of stimulating DNA synthesis, wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ ID NO. 13, when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (8, 9)
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10. An isolated protein δ
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (11, 12)
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the protein subunit is encoded by a DNA molecule comprising a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
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13. An isolated DNA molecule encoding a protein δ
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
SSC, 0.2% SDS at 53°
C. - View Dependent Claims (14, 15, 16, 17)
- subunit of polymerase III holoenzyme, wherein the subunit is capable of stimulating DNA synthesis by the polymerase III holoenzyme wherein the DNA molecule comprises a nucleic acid sequence which hybridizes to a nucleotide sequence corresponding to SEQ. ID. NO. 6 when hybridization is performed in 2×
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18. An isolated DNA molecule consisting of SEQ ID NO. 11 or SEQ ID NO. 12.
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19. An isolated protein consisting of the amino acid sequence encoded by SEQ ID NO. 11 or SEQ ID NO. 12.
Specification
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Current AssigneeAventisub II, Inc. (Sanofi )
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Original AssigneeCornell Research Foundation Incorporated (Cornell University)
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InventorsO'Donnell, Michael E.
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Primary Examiner(s)HUTSON, RICHARD G
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Application NumberUS08/828,323Time in Patent Office2,930 DaysField of Search435/194, 435/183, 435/320.1, 435/252.3, 435/254.11, 435/325, 435/410, 530/350, 530/358, 536/23.2, 536/23.7, 536/23.1, 935/22US Class Current435/194CPC Class CodesC12N 9/1252 DNA-directed DNA polymerase...
