Implementation of microfluidic components in a microfluidic system

• US 6,878,271 B2
• Filed: 12/23/2002
• Issued: 04/12/2005
• Est. Priority Date: 09/09/2002
• Status: Expired due to Fees

• Current Assignee: Cytonome/St, Llc (Cytonome Inc.)
• Original Assignee: Cytonome Inc.
• Inventors: Deshpande, Manish, Gilbert, John Richard
• Primary Examiner(s): Walker, W. L.
• Assistant Examiner(s): MENON, KRISHNAN S
• Application Number:
  US10/329,018
• Publication Number:
  US 20040045891A1
• Time in Patent Office: 841 Days
• Field of Search: 210/321.61, 210/321.75, 210/321.84, 435/287.2, 435/288.1, 435/288, 422/101, 422/102, 216/2
• US Class Current: 210/321.61
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/0666 Solenoid valves
• CPC Class Codes: B01L 3/06 Crystallising dishes
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: C30B 29/58 Macromolecular compounds
• CPC Class Codes: C30B 7/00 Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00

Abstract

A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system.

Claims

1. A microfluidic system, comprising:

a first microchannel formed in a substrate;

a first communication port coupling the first microchannel to a surface of the substrate;

a second microchannel formed in the substrate;

a third microchannel formed in the substrate; and

a capping module having a recess formed therein and having a semi-permeable membrane for separating substances by size exclusion covering the recess to form a chamber, the membrane forming a wall of said chamber, the capping module including an inlet to the chamber and an outlet to the chamber formed therein, wherein the capping module is adapted to be stacked on the substrate, such that the inlet to the chamber is placed in communication with the first microchannel, the outlet to the chamber is placed in communication with the second microchannel, and said membrane is placed in communication with the third microchannel, such that filtrate passing through the membrane enters the third microchannel thereby introducing a microfluidic filtration function into the microfluidic system.

2. The system of claim 1, further comprising a second communication port connecting the second microchannel to a surface of the substrate.

3. A microfluidic system, comprising:

a substrate having a plurality of microchannels formed therein, wherein each microchannel includes one or more communication ports for connecting the microchannel to a surface of the substrate; and

a plurality of microfluidic capping modules, each having associated therewith a semi-permeable membrane for performing a microfluidic filtering function disposed over a recess to form a chamber in the associated capping module, an inlet to the chamber, and an outlet to the chamber, and

wherein a first of said microfluidic capping modules is stacked on the substrate to place one of the inlet and the outlet of the first microfluidic capping module in fluid communication with one or more of the microchannels via a communication port associated with said one or more microchannels to incorporate a first microfluidic filtering function into the system and a second of said microfluidic capping modules is stacked on the substrate to place one of the inlet and the outlet of the second microfluidic capping module in fluid communication with another one or more of the microchannels via a communication port associated with said another one or more of the microchannels to incorporate a second microfluidic filtering function into the system.

4. A microfabricated filtration system, comprising:

a capping module including a membrane for separating a substance into a first and second component;

a first flow path for transmitting a substance through the microfluidic system, wherein the capping module intersects the first flow path when the capping module is stacked on a substrate;

a first reservoir formed in the capping module above the membrane and having an inlet in communication with the first flow path for receiving the substance and having an outlet in communication with the first flow path for conveying the first component from the first reservoir;

a second reservoir defined in the substrate for receiving the second component of the substance;

a second flow path in fluid communication with the second reservoir for receiving the second component of the substance; and

a first flow source in communication with the first fluid path for inducing a flow in the substance, wherein the capping module is stacked on the substrate such that the first reservoir is in communication with the second reservoir and separated from the second reservoir by the membrane, and a substance conveyed along the first flow path is separated into a first component, which passes through the first reservoir and along the first flow path, and a second component, which passes through the membrane and along the second flow path.

5. A microfabricated filtration system, comprising:

a substrate;

a first flow path in the substrate for conveying a substance;

a second flow path formed in the substrate for receiving and conveying a filtered component of the substance;

a recess formed in the substrate in communication with the second flow path;

a cap defining a retentate chamber, the cap stacked on the substrate such that the retentate chamber is placed in communication with the first flow path;

a membrane affixed to the cap to cover the retentate chamber, wherein the membrane is sized for separating the substance into the filtered component and a retained component and is disposed above the recess when the cap is stacked on the substrate to define a filtrate chamber below the membrane, thereby placing the second flow path adjacent to and separated from the first flow path by the membrane;

a third flow path formed in the substrate in communication with the retentate chamber for receiving and conveying the retained component of the substance from the retentate chamber.