Ultra high throughput bioassay screening system

• US 6,878,345 B1
• Filed: 11/23/1998
• Issued: 04/12/2005
• Est. Priority Date: 12/08/1997
• Status: Expired due to Term

• Current Assignee: Douglas Scientific LLC
• Original Assignee: Thomas W. Astle
• Inventors: Astle, Thomas W.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Handy, Dwayne K
• Application Number:
  US09/198,018
• Time in Patent Office: 2,332 Days
• Field of Search: 422/63, 422/66, 422/68.1, 422/82.05, 436/43, 436/44, 436/47, 436/48, 435/286.2, 435/287.1, 435/287.2, 435/287.5, 435/288.3, 435/288.4, 435/288.7, 534/12, 534/15, 534/20, 534/53
• US Class Current: 422/552
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/0265 using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: G01N 2035/023 forming cuvettes in situ, e.g. from plastic strip
• CPC Class Codes: G01N 2035/0436 with pre-packaged reagents, i.e. test-packs
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones
• CPC Class Codes: Y10T 436/110833 Utilizing a moving indicator strip or tape

Abstract

In a preferred embodiment, a method of performing biological assays, including: providing a longitudinally extending carrier tape having thermally formed therein a plurality of reagent receiving wells; adding a reagent to each of said reagent receiving wells; permitting each of said reagent receiving wells to incubate at a predetermined temperature for a predetermined time; and performing a biological analysis on each of said reagent receiving wells.

Claims

1. A method of chemical compound storage, comprising:

(a) providing a longitudinally extending carrier tape having therein two or more matrices of thermoformed chemical receiving wells, each of said two or more matrices being separated longitudinally from an adjacent one of said two or more matrices by a space greater in dimension than a space separating adjacent ones of said chemical receiving wells;

(b) simultaneously adding to each of said chemical receiving wells in one of said two or more matrices a chemical compound; and

(c) then, simultaneously adhering a scaling material around each of said chemical receiving wells in one of said two or more matrices to retain said chemical compounds therein and to minimize evaporation from said chemical receiving wells.

2. A method of chemical compound storage, as defined in claim 1, further comprising:

attaching said sealing material to said carrier tape around each of said chemical receiving wells using a pressure sensitive adhesive.

3. A method of chemical compound storage, as defined in claim 2, further comprising:

forming said carrier tape into a compact roll for storage, said roll having about 100,000 aliquots and dimensions of about 16 inches in diameter by four inches wide.

4. A method of chemical compound storage, as defined in claim 1, further comprising:

providing said carrier tape of a thermoformable material having a thickness on the order of from about 15 mils to about 20 mils.

5. A method of chemical compound storage, as defined in claim 1, further comprising:

providing said carrier tape formed of polypropylene to provide solvent resistance.

6. A method of chemical compound storage, as defined in claim 1, further comprising:

providing said carrier tape formed of clear polycarbonate or polystyrene to facilitate optical reading of contents within said chemical receiving wells.

7. A method of chemical compound storage, as defined in claim 1, further comprising:

providing said chemical receiving wells in said two or more matrices selected from the group consisting of 8×

12 wells with a spacing of 9 mm between centers, 16×

24 wells with a spacing of 4.5 mm between centers, and 32×

48 wells with a spacing of 2.25 mm between centers.

8. A method of chemical compound storage, as defined in claim 7, further comprising:

providing each of said repetitive matrices with a unique identifier.

9. A method of chemical compound storage, as defined in claim 2, further comprising:

removing said sealing material after adhesion, using said pressure sensitive adhesive, to said carrier tape by pulling said sealing material from said carrier tape without the use of a knife structure.

10. A method of chemical compound storage, as defined in claim 1, further comprising:

providing said sealing material removably heat sealed to said carrier tape to permit removal of said sealing material after being adhered to said carrier tape by pulling said sealing material from said carrier tape without the use of a knife structure.

11. A method of chemical compound storage, as defined in claim 10, further comprising providing said seal material as a two layer material having:

(a) a lower, seal layer of a low melting point material inert to the contents of said chemical receiving wells; and

(b) an upper high melting point layer having a higher tensile strength than said seal layer and being joined to said seal layer, to assist in removing said sealing material from said carrier tape.

12. A method of chemical compound storage, as defined in claim 11, further comprising:

providing said lower seal layer formed of a material selected from the group consisting of modified low density polyethylene and ethyl vinyl acetate.

13. A method of chemical compound storage, as defined in claim 11, further comprising:

providing said upper layer formed from polyester.

14. A method of chemical compound storage, as defined in claim 2, further comprising:

removing said sealing material from said carrier tape by using a heated roll to warm said sealing material for removal.

15. A method of chemical compound storage, as defined in claim 2, further comprising:

(a) perforating said carrier tape with holes between said chemical receiving wells, said holes being disposed near upper edges of said chemical receiving wells; and

(b) evacuating-space between said seal material and said carrier tape at time of sealing through said holes to assure an intimate leak tight seal is achieved between said seal material and said carrier tape.

16. A method of chemical compound storage, as defined in claim 2, further comprising:

die cutting said sealing material around one of said two or more matrices of said chemical receiving wells to allow manual removal of said sealing material from said pattern of said chemical receiving wells.

17. A method of chemical compound storage, as defined in claim 3, further comprising:

spinning said roll to force contents of said chemical receiving wells to bottoms of said chemical receiving wells by centrifugal force.

18. A method of chemical compound storage, as defined in claim 1, further comprising:

severing individual said two or more matrices of said chemical receiving wells from said carrier tape so that said individual said two or more matrices can be used independently.

22. A method of chemical compound storage, as defined in claim 1, further comprising:

indexing said two or more matrices of said thermoformed chemical receiving wells using a tractor drive.

19. A device for chemical compound storage, comprising:

a longitudinally extending carrier tape having therein a plurality of thermoformed chemical receiving wells, said chemical wells being disposed in two or more matrices on said carrier tape and a liquid tight sealing material adhered around each of said thermoformed chemical receiving wells to retain said chemical compounds therein and to minimize evaporation of said chemical compounds.

20. A device for chemical compound storage, as defined in claim 19, wherein said liquid tight sealing material is adhered to said carrier tape around each of said chemical receiving wells using a pressure sensitive adhesive.

21. A device for chemical compound storage, as defined in claim 20, wherein:

said carrier tape is formable into a compact roll for storage, said roll having about 100,000 aliquots and dimensions of about 16 inches in diameter by four inches wide.

23. A device for chemical compound storage, as defined in claim 19, further comprising:

adhering said liquid tight sealing material around said chemical receiving wells on said carrier tape using a heat sealed material.