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Device for lysing cells, spores, or microorganisms

  • US 6,878,540 B2
  • Filed: 11/07/2001
  • Issued: 04/12/2005
  • Est. Priority Date: 06/25/1999
  • Status: Expired due to Term
First Claim
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1. A device for use with an ultrasonic transducer to lyse components of a fluid sample, the device comprising:

  • a) a cartridge having;

    i) a lysing chamber;

    ii) an inlet port in fluid communication with the lysing chamber; and

    iii) an outlet port for exit of the sample from the lysing chamber, wherein the inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and wherein the chamber is defined by at least one wall having an external surface to which the ultrasonic transducer may be coupled;

    b) at least one membrane or filter positioned in the lysing chamber for capturing the sample components to be lysed as the sample flows through the chamber; and

    c) beads disposed in the lysing chamber for rupturing the sample components.

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