Automated microfabrication-based biodetector

• US 6,878,755 B2
• Filed: 01/22/2001
• Issued: 04/12/2005
• Est. Priority Date: 01/22/2001
• Status: Expired due to Fees

• Current Assignee: MicroGen Systems, Inc.
• Original Assignee: MicroGen Systems, Inc.
• Inventors: Singh, Angad, Iqbal, Shahzi S.
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US09/766,742
• Publication Number:
  US 20030186218A1
• Time in Patent Office: 1,541 Days
• Field of Search: 422/100, 422/103, 422/82.05, 422/82.07, 422/82.08, 436/172, 436/177, 436/180
• US Class Current: 522/100
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: F04B 43/046 with piezoelectric drive

Abstract

A system, apparatus, and method for processing a sample for chemical and/or biological analysis, and detecting one or more target substances. A first system of microfabricated components includes at least a reservoir and a channel, and a second system of detection components including at least a lens. The lens is focused on a sensing platform of the first system. The sensing platform is coupled to the reservoir by the channel. Various types of detection systems can be utilized with the present invention including fluorescence detection systems with a laser that is positioned to illuminate a sample in the sensing platform. The microfabricated components include one or more pumps, valves, mixers, and filters. A thermoelectric cooler can be positioned to control the temperature of at least one of the microfabricated components. A variety of component configurations can be implemented, and a variety of different processes can be performed, depending on the configuration of components. The device can also be networked with other information processing devices and share data regarding substances detected from the sample.

Claims

1. A biosensor system for processing a sample and detecting one or more target substances in the sample, comprising:

a data processing and control unit;

a microfluidic system couplable to communicate with the data processing and control unit, wherein the microfluidic system includes microfabricated components;

a detection system coupled to receive a processed sample from the microfluidic system and transmit signals regarding the target substances to the data processing and control unit; and

a handheld housing including the data processing and control unit, and the detection system, wherein the data processing and control unit and the detection system are permanently fixed in the housing, and the microfluidic system is insertable and removable from the housing.

2. The system as set forth in claim 1, further comprising a user interface coupled to receive input from a user and provide output to the user, the user interface being further coupled to provide the input from the user to the data processing and control unit.

3. The system as set forth in claim 2, wherein the output to the user includes information regarding the target substances.

4. The system as set forth in claim 2, wherein the input from the user includes information regarding the processing to be performed on the sample.

5. The system as set forth in claim 1, wherein the data processing and control unit processes information from the detection system.

6. The system as set forth in claim 1, wherein the data processing and control unit includes one or more driver units coupled to control operation of the components in the microfluidic system.

7. The system as set forth in claim 1, wherein the data processing and control unit includes one or more driver units coupled to control operation of the detection system.

8. The system as set forth in claim 1, further comprising a thermo-electric cooler for heating and cooling the sample during processing.

9. The system as set forth in claim 1, wherein the microfabricated components include one or more pumps.

10. The system as set forth in claim 9, wherein at least one of the pumps is electro-magnetically actuated.

11. The system as set forth in claim 9, wherein at least one of the pumps is piezoelectrically actuated.

12. The system as set forth in claim 1, wherein the microfabricated components include one or more mixers.

13. The system as set forth in claim 12, wherein the one or more mixers include a nozzle for injecting a first substance into a chamber containing the sample.

14. The system as set forth in claim 1, wherein the microfabricated components include one or more filters.

15. The system as set forth in claim 1, wherein the microfabricated components include one or more valves.

16. The system as set forth in claim 1, wherein the microfabricated components include one or more flow sensors.

17. The system as set forth in claim 1, further comprising an insert detector configured to detect coupling of the microfluidic system to communicate with the data comprising and control unit.

18. The system as set forth in claim 8, further comprising a loading lever operable to place the thermo-electric cooler in contact with the microfluidic system.

19. The system as set forth in claim 16, further comprising a control system operable to compare an actual flow rate to a desired flow rate in the microfluidic system, and to adjust operation of a pump to achieve the desired flow rate.

20. The system as set forth in claim 15, wherein at least one of the valves is formed as a movable flap in a channel in the microfluidic system, one end of the flap being fixed to one side wall of the channel, and another end of the flap being movable between an open position and a closed position.

21. The system as set forth in claim 20, wherein the flap is angularly positioned across the width of the channel, with the end that is closer to the start of the flow being anchored to the one sidewall of the channel.

22. The system as set forth in claim 20, further comprising a first flap positioned in an inlet channel to a chamber, and a second flap position in an outlet channel from the chamber, wherein a vacuum created by movement of a diaphragm pump in one direction over the chamber forces the free end of the second flap into the sidewall of the outlet channel, thereby preventing backflow from the outlet channel into the chamber, and further wherein a vacuum created by movement of the diaphragm pump in another direction over the chamber forces the free end of the first flap into the sidewall of the inlet channel, thereby preventing flow from the inlet channel into the chamber as a substance in the chamber is expelled from the chamber through the outlet channel.

23. The system as set forth in claim 1, wherein the data processing and control unit is configured to communicate with an information network, and data from the data processing and control unit can be accessed from a remote workstation coupled to the network.

24. The system as set forth in claim 17, wherein a signal from the insert detector is used to start operating other components in the system.

25. The system as set forth in claim 1, wherein the detection system is operable to detect electrical signals from a processed sample.

26. The system as set forth in claim 1, wherein the detection system is operable to detect fluorescence of a processed sample.