Core-shell particle including signal-generating substance enclosed therein and process for producing the same

• US 6,881,484 B2
• Filed: 05/30/2001
• Issued: 04/19/2005
• Est. Priority Date: 05/30/2001
• Status: Expired due to Fees

• Current Assignee: Mitsubishi Chemical Medience Corporation (Mitsubishi Chemical Group Corp.), NanoCarrier Co., Ltd.
• Original Assignee: Mitsubishi Kagaku Iatron, Inc., NanoCarrier Co., Ltd.
• Inventors: Kataoka, Kazunori, Nagasaki, Yukio, Hoshino, Nobuhiro, Shibata, Naoya
• Primary Examiner(s): LE, HOA T
• Application Number:
  US10/479,247
• Publication Number:
  US 20040171771A1
• Time in Patent Office: 1,420 Days
• Field of Search: 428/407, 525/902
• US Class Current: 428/407
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10S 525/902 Core-shell
• CPC Class Codes: Y10T 428/2998 including synthetic resin or polymer

Abstract

A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of (1) a core portion substantially made of a water-insoluble polymer compound; and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer, characterized in that the signal-generating substance is included in the core portion, is disclosed, and a process for producing the same is also disclosed.

The core-shell particle including a signal-generating substance therein is useful as a labeling substance in which neither radioisotopes having handling restrictions nor unstable enzymes are required, a high sensitivity superior to fluorescent substances is achieved, and nonspecific absorption does not occur.

Claims

1. A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of

(1) a core portion substantially made of a water-insoluble polymer compound;

and

(2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and

the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer,

characterized in that the signal-generating substance is included in the core portion.

2. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the water-soluble polymer compound is polyethyleneglycol.

3. The core-shell particle including a signal-generating substance therein according to claim 2, wherein the water-insoluble polymer compound is polystyrene.

4. The core-shell particle including a signal-generating substance therein according to claim 2, wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.

5. The core-shell particle including a signal-generating substance therein according to claim 2, wherein the signal-generating substance is a dye.

6. The core-shell particle including a signal-generating substance therein according to claim 2, wherein the signal-generating substance is a fluorescent substance.

7. The core-shell particle including a signal-generating substance therein according to claim 2, wherein the signal-generating substance is a lanthanoid chelate.

8. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the water-insoluble polymer compound is polystyrene.

9. The core-shell particle including a signal-generating substance therein according to claim 8, wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.

10. The core-shell particle including a signal-generating substance therein according to claim 8, wherein the signal-generating substance is a dye.

11. The core-shell particle including a signal-generating substance therein according to claim 8, wherein the signal-generating substance is a fluorescent substance.

12. The core-shell particle including a signal-generating substance therein according to claim 8, wherein the signal-generating substance is a lanthanoid chelate.

13. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.

14. The core-shell particle including a signal-generating substance therein according to claim 13, wherein the signal-generating substance is a dye.

15. The core-shell particle including a signal-generating substance therein according to claim 13, wherein the signal-generating substance is a fluorescent substance.

16. The core-shell particle including a signal-generating substance therein according to claim 13, wherein the signal-generating substance is a lanthanoid chelate.

17. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the signal-generating substance is a dye.

18. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the signal-generating substance is a fluorescent substance.

19. The core-shell particle including a signal-generating substance therein according to claim 1, wherein the signal-generating substance is a lanthanoid chelate.

20. A process for producing the core-shell particle including a signal-generating substance therein according to claim 1, characterized by immersing

(a) a core-shell particle consisting of (1) a core portion substantially made of a water-insoluble polymer compound;

and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush;

the core portion and the shell portion being a block copolymer of a water-insoluble polymer and a water-soluble polymer as a whole, and

(b) a signal-generating substance in a solution containing an organic solvent capable of swelling the water-insoluble polymer compound, to include the signal-generating substance in the core portion.