Method for detecting α-oxoaldehydes in the whole blood, blood plasma and/or serum of a patient

• US 6,884,223 B2
• Filed: 05/29/2001
• Issued: 04/26/2005
• Est. Priority Date: 06/09/2000
• Status: Expired due to Fees

• Current Assignee: Institut Fur Chemo- Und Biosensorik Munster e.V.
• Original Assignee: Institut Fur Chemo- Und Biosensorik Munster e.V.
• Inventors: Kleibohmer, Wolfgang, Schulze-Pellengahr, Uta
• Primary Examiner(s): Jones, Mary Beth
• Assistant Examiner(s): Mallari, Patricia C.
• Application Number:
  US10/297,424
• Publication Number:
  US 20030176805A1
• Time in Patent Office: 1,428 Days
• Field of Search: 600/300, 600/322, 600/348, 600529-543, 73/233, 422 83- 85, 422/50, 422/51, 422/61, 422/68.1, 422/9.1, 422/9.6, 422/9.7, 422/9.8, 436/815, 436/900, 436/128
• US Class Current: 600/532
• CPC Class Codes: A61B 5/082 Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath A61B5/083, A61B5/091 take precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72

Abstract

A method for detecting α-oxoaldehydes in the whole blood, blood plasma and/or serum of a patient. A respiratory air sample is analyzed for the presence of at least one α-oxoaldehyde, and the presence of α-oxoaldehydes in the whole blood, blood plasma and/or serum of the patient is determined from the results of the analysis.

Claims

1. A method for detecting α

-oxoaldehydes in at least one of whole blood, blood plasma and serum of a patient, the method comprising analyzing a breath air sample for the presence of at least one α

-oxoaldehyde and deducing the presence of α

-oxoaldehydes in the at least one of whole blood, blood plasma and serum of the patient from the analysis result.

2. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

3. The method according to claim 2 wherein determining the resulting derivatives includes determining the resulting derivatives by one of the following methods fluorometrically;

photometrically; and

, chromatographically.

4. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes at least one of enclosing the breath air sample in a sample bag, enclosing the breath air sample in another suitable sample container and directing the breath air sample through an impinger.

5. The method according to claim 4 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

6. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde and deducing the presence of α

-oxoaldehydes in the at least one of whole blood, blood plasma and serum of the patient from the analysis result includes converting methylglyoxal which is contained in the breath air sample into lactate with glyoxalase and subsequently detecting the lactate with a sensor.

7. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes detecting the α

-oxoaldehydes by at least one of the following methods;

electrochemically;

spectrometrically;

enzymatically; and

, immunochemically.

8. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes determining the α

-oxoaldehydes in the breath air sample mass-spectrometrically.

9. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes determining the concentration of α

-oxoladehydes in the breath air sample, and deducing the presence of α

-oxoaldehydes in the at least one of whold blood, blood plasma and serum of the patient includes deducing the concentration of α

-oxoaldehydes in the at least one of whole blood, blood plasma and serum of the patient therefrom.

10. The method according to claim 9 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes analyzing a breath air sample for the presence of at least one of methylglyoxal, glyoxal and 3-deoxyglucuron.

11. The method according to claim 10 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes at least one of enclosing the breath air sample in a sample bag, enclosing the breath air sample in another suitable sample container and directing the breath air sample through an impinger.

12. The method according to claim 11 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

13. The method according to claim 10 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

14. The method according to claim 9 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

15. The method according to claim 9 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes at least one of enclosing the breath air sample in a sample bag, enclosing the breath air sample in another suitable sample container and directing the breath air sample through an impinger.

16. The method according to claim 15 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

17. The method according to claim 1 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes analyzing a breath air sample for the presence of at least one of methylglyoxal, glyoxal and 3-deoxyglucuron.

18. The method according to claim 17 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes at least one of enclosing the breath air sample in a sample bag, enclosing the breath air sample in another suitable sample container and directing the breath air sample through an impinger.

19. The method according to claim 18 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.

20. The method according to claim 17 wherein analyzing a breath air sample for the presence of at least one α

-oxoaldehyde includes derivatizing the at least one α

-oxoaldehyde in the breath air sample by means of a derivatization medium and determining the resulting derivatives.