Genes differentially expressed in secretory versus proliferative endometrium

• US 6,884,578 B2
• Filed: 12/11/2000
• Issued: 04/26/2005
• Est. Priority Date: 03/31/2000
• Status: Expired due to Term

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Warrington, Janet A., Mahadevappa, Mamatha
• Primary Examiner(s): Myers, Carla J.
• Assistant Examiner(s): Spiegler, Alexander H.
• Application Number:
  US09/734,752
• Publication Number:
  US 20010044104A1
• Time in Patent Office: 1,597 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/7.1, 435/69.1, 536/23.1, 536/23.5, 536/24.3, 536/23.4, 536/24.1, 436/501
• US Class Current: 435/6.14
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The present invention compares expression profiles from matched samples to identify differential gene expression. Samples are matched according to physiological, pharmacological and/or disease state. Comparison of matched samples eliminates gene expression differences that are the result of changes in variables that are not of interest. The gene expression differences that remain can be attributed with a high degree of confidence to the unmatched variation. The gene expression differences thus identified can be used for example to diagnose disease, identify physiological state, design drugs, and monitor therapies.

Claims

1. A method for diagnosing endometrial cancer in an endometrial tissue sample comprising:

obtaining a gene expression profile from the endometrial tissue sample wherein expression of the following genes is measured;

KIAA0367, KIAA0119, platelet activating factor acetylhydrolase 1B gamma-subunit, UDP-galactose transporter related ioszyme, HMG-1 and Lamin B; and

identifying the sample as being cancerous if KIAA0367 expression is downregulated as compared to a normal control sample and KIAA0119, platelet activating factor acetylhydrolase 1B gamma-subunit, UDP-galactose transporter related ioszyme, HMG-1 and Lamin B expression is upregulated as compared to a normal control sample.