Fabricating biopolymer arrays

• US 6,884,580 B2
• Filed: 11/26/2001
• Issued: 04/26/2005
• Est. Priority Date: 04/30/1999
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Caren, Michael P., Schembri, Carol T., Webb, Peter G.
• Primary Examiner(s): Siew, Jeffrey
• Application Number:
  US10/059,957
• Publication Number:
  US 20020064889A1
• Time in Patent Office: 1,247 Days
• Field of Search: 435/6, 435/7.1, 435/91.1, 435/91.2, 435/287.2, 536/22.1, 536/23.1, 536/273, 536 2431- 2433
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A method and apparatus for fabricating an array of biopolymers on a substrate using a biopolymer or biomonomer fluid, and using a dispensing head. The head has a reservoir chamber and at least one jet which can dispense droplets onto a substrate. The jet includes a capillary delivery chamber communicating with the reservoir chamber, which delivery chamber has an orifice. The jet further includes an ejector which, when activated, causes a droplet to be ejected from the orifice. The method includes loading the head by positioning the head with the orifice adjacent and facing a biomonomer or biopolymer fluid, and providing a load pressure to the reservoir chamber. The load pressure is sufficiently negative such that the fluid is drawn into the reservoir chamber through the orifice and delivery chamber, while simultaneously being insufficient to result in ambient atmosphere entering the delivery chamber through the orifice once the head has been loaded and no further fluid is facing and adjacent the orifice. The head is positioned with the orifice facing the substrate and multiple droplets are dispensed from the head orifice so as to form an array of droplets on the substrate.

Claims

1. A method of fabricating an array of biopolymers on a substrate using a dispensing head with biopolymer or biomonomer fluids, the fluid dispensing head having:

a reservoir chamber;

multiple jets which can dispense droplets onto a substrate, each jet including a delivery chamber communicating with the reservoir chamber, and including an orifice and an ejector which, when activated, causes a droplet to be ejected from the orifice;

the method comprising;

(a) loading the head through orifices of the jets with biopolymer or biomonomer fluids;

(b) positioning the head with the orifices facing the substrate;

(c) dispensing multiple droplets from the head orifices so as to form an array of droplets on the substrate;

(d) positioning the head with the orifices facing a cleaning station which is spaced from the substrate;

(e) exposing the head about the orifices to a cleaning fluid from the cleaning station; and

(f) repeating (a) to (f) as needed so as to form the array.

2. A method according to claim 1 wherein the cleaning station comprises a pad carrying cleaning fluid and the head is exposed to the cleaning fluid by wiping at least one of the head and pad across the other.

3. A method according to claim 1 wherein the fluid is a polynucleotide containing fluid.

4. A method according to claim 1 wherein the fluid is a polymer of amino acids.

5. A method according to claim 1 wherein the fluids are polynucleotide containing fluids.

6. A method according to claim 1 wherein the fluids contain amino acid polymers.