Multifunctional polymeric surface coatings in analytic and sensor devices
First Claim
1. A method for reducing non-specific adsorption of inorganic ions, peptides, proteins, and saccharides to a surface of a device comprisingapplying to or coating onto a surface of an analytical or sensing device, wherein the surface comprises a material selected from the group consisting of metals, metal oxides, and charged polymers. a non-interactive polyionic multifunctional copolymer, wherein the non-interactive polyionic multifunctional copolymer comprises non-interactive polymer sidechains covalently grafted onto a charged polyionic polymeric backbone which has an anionic charge at a pH greater than 4, wherein the polyionic polymeric backbone interacts with the surface at a pH greater than 4, where some or all of the non-interactive polymer sidechains are partially or fully functionalized at or near the free terminal position of the non-interactive polymer sidechains with a functional molecule.
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Abstract
Multifunctional, polyionic copolymers with molecular architectures and properties optimized for specific applications are synthesized on/or applied to substrate surfaces for analytical and sensing purposes. The coatings are particularly useful for suppression of non-specific interaction, adsorption or attachment of molecular or ionic components present in an analyte solution. Chemical, biochemical or biological groups that are able to recognize, interact with and bind specifically to target molecules in the material containing the analyte to be detected can be coupled to, integrated into, or absorbed to the multifunctional copolymers. These multifunctional copolymer coatings are compatible with a variety of different established methods to detect, sense and quantify the target molecule in an analyte. The multifunctional copolymer coatings typically include brush copolymers based on a polycationic or polyanionic (jointly referred to herein as ‘polyionic’) backbone with side chains that control interaction with the environment, such as poly(ethylene glycol) or poly(ethylene oxide)-based side chains that decrease cellular adhesion, and analyte-specific side chains. They can be used to pattern the surfaces into non-adhesive and specifically adhesive areas by applications of known techniques such as microfluidic or contact printing techniques.
263 Citations
13 Claims
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1. A method for reducing non-specific adsorption of inorganic ions, peptides, proteins, and saccharides to a surface of a device comprising
applying to or coating onto a surface of an analytical or sensing device, wherein the surface comprises a material selected from the group consisting of metals, metal oxides, and charged polymers. a non-interactive polyionic multifunctional copolymer, wherein the non-interactive polyionic multifunctional copolymer comprises non-interactive polymer sidechains covalently grafted onto a charged polyionic polymeric backbone which has an anionic charge at a pH greater than 4, wherein the polyionic polymeric backbone interacts with the surface at a pH greater than 4, where some or all of the non-interactive polymer sidechains are partially or fully functionalized at or near the free terminal position of the non-interactive polymer sidechains with a functional molecule.
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5. A method for making a biosensing or analytical device having reduced non-specific adsorption of inorganic ions, peptides, proteins and saccharides comprising
applying to or coating onto a surface of the biosensing or analytical device non-interactive polyionic multifunctional copolymers, wherein the non-interactive polyionic multifunctional copolymers comprise non-interactive polymer sidechains covalently grafted onto a charged polyionic polymeric backbone, and wherein the surface comprises a material selected from the group consisting of metals, metal oxides, and charged polymers, and the surface of the device has a charge opposite to the charge of the charged polyionic polymeric backbone, and wherein some of the non-interactive polymer side chains comprise a functional moiety and some of the non-interactive polymer side chains do not comprise a functional moiety.
Specification