System for controlling the flow of a fluid through a substrate

• US 6,886,409 B2
• Filed: 03/11/2002
• Issued: 05/03/2005
• Est. Priority Date: 03/13/2001
• Status: Expired due to Fees

• Current Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Original Assignee: PamGene International BV (VitrOmics Healthcare Holding BV)
• Inventors: Carpay, Wilhelmus Marinus
• Primary Examiner(s): Lefkowitz, Edward
• Assistant Examiner(s): JENKINS, JERMAINE L
• Application Number:
  US10/095,834
• Publication Number:
  US 20030000311A1
• Time in Patent Office: 1,149 Days
• Field of Search: 73700-756, 731/521.8, 731/523.9, 731/524.2, 731/525.1, 731/95, 738/612.4
• US Class Current: 73/716
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums

Abstract

A system is described for controlling the flow of a sample fluid through a substrate having first and second surfaces and at least one area with a plurality of through-going capillary channels. The system comprises a housing having a chamber for receiving the substrate and a pressure differential generator capable of generating and maintaining a pressure difference over the substrate.

Claims

1. A system for controlling the flow of a fluid through a substrate having first and second surface and at least one area with a plurality of through-going capillary channels, characterized by a housing having a chamber for receiving the substrate, means for generating a pressure difference over the substrate to transport the fluid from the first to the second surface and vice versa through the channels of said at least one area, and means for maintaining the pressure difference at a controlled level during the transport of the fluid through the channels.

2. The system of claim 1, wherein the fluid is a sample fluid.

3. The system of claim 1, wherein the maintaining means comprises means for setting a desired level of the pressure difference.

4. The system of claim 3, wherein the means for generating a pressure difference comprise means to change the volume of the chamber, wherein the maintaining means comprises a pressure measuring device and a control device to operate the volume changing means.

5. The system if claim 3, comprising means for monitoring the operation of the maintaining means, wherein said monitoring means are adapted to change the pressure difference over the substrate to transport the fluid in the reverse direction through the channels as soon as the operation of the maintaining means shows that the fluid has been transported through the substrate.

6. The system of claim 1, wherein the means for generating a pressure difference comprise means to change the volume of the chamber, wherein the maintaining means comprises a pressure measuring device and a control device to operate the volume changing means.

7. The system of claim 6, wherein the monitoring means monitor the operation of said maintaining means, wherein the pressure difference is changed to transport the fluid in reverse direction as soon as the pressure difference remains at a constant level without operation of the maintaining means.

8. The system of claim 6, wherein the volume changing means comprises a cylinder piston assembly connected to the chamber and wherein said control device operates the piston of the cylinder piston assembly.

9. The system of claim 6, wherein the pressure difference is changed in the reverse direction by reversing the change of volume.

10. The system of claim 6, further comprising means for measuring the change of volume required to transport the fluid through the channels from the first to the second surface or vice versa, wherein said measuring means is adapted to indicate if said change of volume is varying in successive transports of the fluid through the channels.

11. The system of claim 10, comprising means to input the initial volume of a sample fluid, wherein said measuring means is adapted to compare said initial volume and said change of volume, wherein said measuring means provides an indication if a difference is established.

12. The system of claim 6, comprising means for measuring the time required to transport the fluid through the channels from the first to the second surface or vice versa, wherein said measuring means is adapted to indicate if the measured time indicates a variation of fluid flow rate.

13. The system of claim 6, comprising a washing device having washing fluid feed and discharge tubes wherein the washing device is adapted to supply and remove washing fluid to and from the chamber as controlled by the maintaining means.

14. The system if claim 1, comprising means for monitoring the operation of the maintaining means, wherein said monitoring means are adapted to change the pressure difference over the substrate to transport the fluid in the reverse direction through the channels as soon as the operation of the maintaining means shows that the fluid has been transported through the substrate.

15. The system of claim 14, wherein the monitoring means monitor the operation of said maintaining means, wherein the pressure difference is changed to transport the fluid in reverse direction as soon as the pressure difference remains at a constant level without operation of the maintaining means.

16. The system of claim 14, wherein the monitoring means comprises a cylinder piston assembly connected to the chamber and wherein said monitoring means operates the piston of the cylinder piston assembly.

17. The system of claim 14, wherein the pressure difference is changed in the reverse direction by reversing the change of volume.

18. The system of claim 14, further comprising means for measuring the change of volume required to transport the fluid through the channels from the first to the second surface or vice versa, wherein said measuring means is adapted to indicate if said change of volume is varying in successive transports of the fluid through the channels.

19. The system of claim 14, comprising means for measuring the time required to transport the fluid through the channels from the first to the second surface or vice versa, wherein said measuring means is adapted to indicate if the measured time indicates a variation of fluid flow rate.

20. The system of claim 14, comprising a washing device having washing fluid feed and discharge tubes wherein the washing device is adapted to supply and remove washing fluid to and from the chamber as controlled by the maintaining means.

21. The system of claim 1, wherein a binding substance is bound to the substrate.

22. The system of claim 1, wherein the substrate is an electrochemically manufactured metal oxide membrane.

23. The system of claim 22, wherein the metal oxide membrane is an aluminum oxide membrane.