Microarrays and their manufacture

• US 6,887,701 B2
• Filed: 06/14/2001
• Issued: 05/03/2005
• Est. Priority Date: 07/30/1999
• Status: Expired due to Fees

• Current Assignee: Large Scale Proteomics Corporation
• Original Assignee: Large Scale Proteomics Corporation
• Inventors: Anderson, N. Leigh, Anderson, Norman G.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/880,019
• Publication Number:
  US 20010041339A1
• Time in Patent Office: 1,419 Days
• Field of Search: 385/12, 385/115, 385/123, 356/244, 422/158, 422/57, 422/82.02, 422/82.11, 435/7.1, 435/287.1, 435/287.2, 435/288.7, 435/808, 435/6, 476/164, 476/165, 476/172, 476/518, 476/527, 476/535, 476/805, 476/809
• US Class Current: 435/287.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00524 in the shape of fiber bundles
• CPC Class Codes: B01J 2219/00547 Bar codes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B01L 3/50857 : using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 2500/00 : Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/5436 : with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 435/808 : Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 442/20 : Coated or impregnated woven, knit, or nonwoven fabric which is not [a] associated with another preformed layer or fiber layer or, [b] with respect to woven and knit, characterized, respectively, by a particular or differential weave or knit, wherein the coating or impregnation is neither a foamed material nor a free metal or alloy layer

Abstract

The microarrays of the present invention are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.

Claims

1. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other with the fibers having different agents of interest immobilized therein or thereon over a length of each fiber and wherein each fiber contains one or more solid phases immobilizing said agents of interest wherein said solid phase being immobilized in or on the fibers.

2. The fiber bundle according to claim 1 wherein said solid phases are particles or thread-like structures embedded in the fibers.

3. A cross-section of the fiber bundle of claim 1 containing a portion of each fiber in the fixed position and the agent of interest from each fiber.

4. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other with the fibers having different agents of interest immobilized therein or thereon over a length of each fiber and wherein at least one of the fibers contains a dye.

5. A cross-section of the fiber bundle of claim 4 containing a portion of each fiber in the fixed position and the agent of interest from each fiber.

6. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other with the fibers having different agents of interest immobilized therein or thereon over a length of each fiber wherein at least some of the fibers contains a mixture of plural agents of interest and different fibers contain a different mixture of plural agents of interest.

7. A cross-section of the fiber bundle of claim 6 containing a portion of each fiber in the fixed position and the agent of interest from each fiber.

8. A fiber bundle comprising a plurality of hollow fibers attached to each other in a fixed position with respect to each other with the fibers having different agents of interest immobilized on the inside of the hollow fibers over a length of each fiber.

9. A cross-section of the fiber bundle of claim 8 containing a portion of each fiber in the fixed position and the agent of interest from each fiber.

10. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other with the fibers having different biological cells immobilized therein or thereon over a length of each fiber.

11. A cross-section of the fiber bundle of claim 10 containing a portion of each fiber in the fixed position and the biological cell from each fiber.

12. A cross-section of a fiber bundle less than about 50 microns thick and containing a portion of each fiber in the fixed position and containing the agent of interest from each fiber wherein the fiber bundle comprises a plurality of fibers attached to each other in a fixed position with respect to each other with the fibers having different agents of interest immobilized therein or thereon over a length of each fiber.