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Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells

  • US 6,887,706 B2
  • Filed: 10/03/2001
  • Issued: 05/03/2005
  • Est. Priority Date: 10/03/2001
  • Status: Expired due to Term
First Claim
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1. A method of differentiating primate embryonic stem cells into neural precursor cells, comprising the steps of:

  • (a) obtaining a primate embryonic stem cell culture, (b) propagating the stem cells, wherein embryoid bodies are formed, and (c) culturing the embryoid bodies in a medium consisting essentially of DMEM/F12, insulin, transferrin, progesterone, putrescine, sodium selenite, heparin and an effective amount of fibroblast growth factor 2, wherein neural precursor cells are generated and wherein the neural precursor cells form rosette formations.

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