Process for typing of HCV isolates

• US 6,887,985 B2
• Filed: 07/06/2001
• Issued: 05/03/2005
• Est. Priority Date: 11/27/1992
• Status: Expired due to Fees

• Current Assignee: Innogenetics NV (H.U. Group Holdings, Inc.)
• Original Assignee: Innogenetics NV (H.U. Group Holdings, Inc.)
• Inventors: Maertens, Geert, Stuyver, Lieven, Van Heuverswyn, Hugo, Rossau, Rudi
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US09/899,302
• Publication Number:
  US 20020168626A1
• Time in Patent Office: 1,397 Days
• Field of Search: 536/23.1, 536/24.3, 435/5, 435/6
• US Class Current: 536/23.1
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/707 non-A, non-B Hepatitis, excluding hepatitis D
• CPC Class Codes: C12Q 2525/101 incorporating non-naturally occurring nucleotides, e.g. inosine
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2563/131 the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin

Abstract

The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of:

• • contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes,
  • detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.

Claims

1. An isolated polynucleic acid specifically hybridizing with a sequence selected from the group consisting of SEQ ID NO:

55 to SEQ ID NO;

81, under conditions allowing discrimination of up to 1 nucleotide mismatch, or the complement thereof.

2. An isolated HCV virus characterized by any of the 5′

UTR nucleotide sequences selected from the group consisting of SEQ ID NO;

55 to 81.