Sensor for performing surface enhanced Raman spectroscopy and method for achieving same

• US 6,888,629 B1
• Filed: 06/25/2001
• Issued: 05/03/2005
• Est. Priority Date: 06/14/2000
• Status: Expired due to Fees

• Current Assignee: The Government Of The United States Of America, As Represented By The Secretary Of The Navy
• Original Assignee: the united states of america as represented by the secretary of the navy
• Inventors: Boss, Pamela A., Lieberman, Stephen H.
• Primary Examiner(s): Nguyen, Tu T.
• Application Number:
  US09/888,737
• Time in Patent Office: 1,408 Days
• Field of Search: 356300-309, 356 36- 50, 600315-323, 600/600
• US Class Current: 356/301
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C03C 17/38 at least one coating being a coating of an organic material
• CPC Class Codes: C03C 2204/08 Glass having a rough surface
• CPC Class Codes: C03C 2217/77 Coatings having a rough surface
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 21/8507 Probe photometers, i.e. with optical measuring part dipped into fluid sample
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/552 Glass or silica

Abstract

A sensor for performing surface enhanced Raman spectroscopy comprises: a) a sensor body having a throughbore; an optical energy source for generating an optical excitation signal; b) a surface enhanced Raman scattering structure that is mounted to the sensor body through which the optical excitation signal is directed for irradiating an analyte, whereupon the analyte generates primary Raman emissions in response to being irradiated by the optical excitation signal, and wherein the surface enhanced Raman scattering structure generates secondary Raman emissions when irradiated by the optical excitation signal; c) an optical detector for generating an output signal that represents the spectral characteristics of the primary and secondary Raman emissions in response to receiving the primary and second Raman emissions; and d) a processor for substantially filtering the secondary Raman emission from the primary Raman emissions and for generating an output signal representing the analyte.

Claims

1. A sensor for performing surface enhanced Raman spectroscopy, comprising:

a sensor body having a throughbore;

an optical energy source for generating an optical excitation signal;

a surface enhanced Raman scattering structure that is mounted to said sensor body through which said optical excitation signal is directed for irradiating an analyte, whereupon said analyte generates primary Raman emissions in response to being irradiated by said optical excitation signal, and wherein said surface enhanced Raman scattering structure generates secondary Raman emissions when irradiated by said optical excitation signal;

an optical detector for generating an output signal that represents spectral characteristics of said primary and secondary Raman emissions in response to receiving said primary and secondary Raman emissions; and

a processor for substantially filtering said secondary Raman emission from said primary Raman emissions and for generating an output signal representing said analyte.

2. The sensor of claim 1 which further includes a first optical fiber for directing said optical excitation signal through said SERS structure.

3. The sensor of claim 2 which further includes a bandpass filter for attenuating any self excited Raman emissions that may be stimulated by said optical excitation signal in said first optical fiber.

4. The sensor of claim 1 further including a second optical fiber for directing said primary and secondary Raman emissions to said optical detector.

5. The sensor of claim 1 further including a long pass filter for filtering optical signals having wavelengths less than a predetermined wavelength.

6. The sensor of claim 1 further including a display for presenting human readable indicia representing said analyte.

7. A sensor for performing surface enhanced Raman spectroscopy, comprising:

a sensor body;

an optical energy source for generating an optical excitation signal;

a surface enhanced Raman scattering structure that is mounted to said sensor body through which said optical excitation signal is directed for irradiating an analyte, whereupon said analyte generates primary Raman emissions in response to being irradiated by said optical excitation signal, and wherein said surface enhanced Raman scattering structure generates secondary Raman emissions when irradiated by said optical excitation signal;

an optical detector for generating an output signal that represents spectral characteristics of said primary and secondary Raman emissions in response to receiving said primary and second Raman emissions; and

a processor for creating a sample file that represents said spectral characteristics of said primary and secondary Raman emissions, a reference file that represents said secondary Raman emissions, and a data file that represents the difference between said sample file and said reference file, and for generating an output signal that represents said analyte where said analyte has spectral characteristics represented by said data file.

8. A method for identifying an analyte using Raman spectroscopy, comprising the steps of:

generating an excitation light signal;

directing said excitation light signal through a SERS structure that is in contact with an analyte so that said analyte generates primary Raman spectral emissions when irradiated by said optical excitation signal, and wherein said SERS structure generates secondary Raman spectral emissions in response to being irradiated by said excitation light signal;

generating an output signal representing said first and second Raman spectral emissions in response to detecting said first and second Raman spectral emissions;

substantially filtering said secondary Raman emission from said primary Raman emissions to create a data file;

identifying one or more candidate analytes characterized by

said primary Raman emissions from said data file; and

generating an output signal that represents said candidate analytes.