Flow cytometry for high throughput screening
First Claim
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1. A flow cytometry apparatus for the detection of particles from a plurality of samples comprising:
- means for moving the plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream, said means for moving the plurality of samples comprising a pump;
means for introducing a separation gas between each of said plurality of samples in said fluid flow stream; and
a flow cytometer for hydrodynamically focusing said fluid flow stream and selectively analyzing said particles in each of said plurality of samples as said fluid flow stream passes through said flow cytometer.
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Abstract
The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
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Citations
34 Claims
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1. A flow cytometry apparatus for the detection of particles from a plurality of samples comprising:
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means for moving the plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream, said means for moving the plurality of samples comprising a pump;
means for introducing a separation gas between each of said plurality of samples in said fluid flow stream; and
a flow cytometer for hydrodynamically focusing said fluid flow stream and selectively analyzing said particles in each of said plurality of samples as said fluid flow stream passes through said flow cytometer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A flow cytometry apparatus for the detection of particles from a plurality of samples, comprising:
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a pump for moving the plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream;
separation means for introducing a parameter-controlled separation gas between each of said plurality of samples in said fluid flow stream; and
a flow cytometer for hydrodynamically focusing said fluid flow stream and selectively analyzing said particles in each of said plurality of samples as said fluid flow stream passes through said flow cytometer, said separation gas having parameters of predetermined values to permit the hydrodynamic focusing of said fluid flow stream in said flow cytometer, said separation means including tubing of a given size for introducing separation gas aliquots of a controlled volume into said fluid flow stream with said samples flowing at a specific rate, said given size and said controlled volume and said specific rate all being predetermined to permit the hydrodynamic focusing of said fluid flow stream in said flow cytometer. - View Dependent Claims (30, 31, 32, 33, 34)
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Specification