Array fabrication

• US 6,890,760 B1
• Filed: 07/31/2000
• Issued: 05/10/2005
• Est. Priority Date: 07/31/2000
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies Europe BV (Agilent Technologies, Inc.)
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Webb, Peter G.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): QUAN, ELIZABETH S
• Application Number:
  US09/628,470
• Time in Patent Office: 1,744 Days
• Field of Search: 347/43, 347/40, 347/78, 347/19, 347/23, 347/14, 347/9, 347/12, 422 63- 67, 422 94-104, 700/241, 700/266, 700/267, 700/283, 436/180
• US Class Current: 436/180
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00475 Sheets
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00695 Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Methods, apparatus, and computer program, for use in fabricating a chemical array. The method may use an apparatus which includes a head system, a transport system, and a processor. The head system has multiple groups of drop dispensers which move in unison, each group having multiple dispensers. The transport system can move the head system with respect to a substrate with different dispensers of the groups following respective paths. The processor can dispense droplets from dispensers during operation of the transport system, in a pattern along a selected path for each group so as to form the array. The method includes identifying an error in one or more dispensers. When a dispenser of a first group is in error, then a second dispenser of each group is moved along the selected path for its group while dispensing droplets from at least the second dispenser of the first group in at least part of the pattern for the selected path of the first group.

Claims

1. An apparatus for fabricating a chemical array, comprising:

(a) a head system with multiple groups of drop dispensers which move in unison, wherein each group comprises a series of drop dispensers, and each series comprises at least a first and a second drop dispenser loaded with a same fluid;

(b) a transport system to move the head system with respect to a substrate with different dispensers in the series of each group following respective paths; and

(c) a processor which;

dispenses droplets from a single dispenser of each group of dispensers in a pattern along a selected path for each group during operation of the transport system, so as to form the array; and

when an error in said first drop dispenser of a series is detected, dispenses droplets from said second dispenser of the series along the selected path for its group.

2. An apparatus according to claim 1 wherein the dispensers are pulse jets.

3. An apparatus according to claim 1 wherein the processor, when the error indication is identified, dispenses droplets from each second dispenser of the series within each of multiple groups in at least part of the pattern for the selected path of the group containing that series.

4. An apparatus according to claim 3 wherein:

a column of dispensers within a group communicate with a corresponding common reservoir for the column.

5. An apparatus according to claim 3 wherein, when the error indication is identified, the processor dispenses droplets from the second dispenser of a series in a complete pattern for the first dispenser of the same series.

6. An apparatus according to claim 3 wherein, when the error indication is identified, the processor dispenses droplets from at least one second dispenser of the first group in a complete pattern for the selected path of the first group.

7. A method according to claim 3 wherein when another error indication is identified by the processor additionally indicating an error in the second dispenser of a second group, the processor alternately moves the first and second dispensers of each group along the selected path for that group while dispensing droplets from non-error dispensers of the first and second groups in different parts of the pattern for the selected path for the first group.

8. A computer program product for use with an apparatus for fabricating a chemical array having:

a head system with multiple groups of drop dispensers which move in unison, wherein each group comprises a series of drop dispensers, and wherein said series comprises at least a first and a second drop dispenser loaded with a same fluid;

a transport system to move the head system with respect to a substrate with different dispensers of the series of each group following respective paths; and

a processor;

the computer program product comprising a computer readable storage medium having a computer program stored thereon which, when loaded into the processor, performs the steps of;

a) dispensing droplets from a single dispenser of each group of dispensers in a pattern along a selected path for each group during operation of the transport system

b) identifying for an error in one or more dispensers of a group; and

c) moving said group of dispensers to dispense droplets from said second drop dispenser of said group along at least part of the path selected for said group.

9. A computer program product according to claim 8 wherein in step (b) droplets are dispensed from each second dispenser of the series within each of multiple groups in at least part of the pattern for the selected path of the group containing that series.

10. A computer program product according to claim 8 wherein when a dispenser of a second set of the first group is additionally identified as being in error, the program causes the first and second dispensers of each group to be alternately moved along the selected path for that group while droplets are dispensed from non-error dispensers of the first and second groups in different parts of the pattern for the selected path for the first group.

11. An apparatus for fabricating a chemical array, comprising:

(a) a head system with multiple groups of drop dispensers which move in unison, wherein each group comprises multiple rows and columns of dispensers and wherein at least two dispensers of a same column within a group are loaded with a same fluid;

(b) a transport system to move the head system with respect to a substrate with different rows following respective paths; and

(c) a processor which;

dispenses droplets from dispensers during operation of the transport system, in a pattern along a selected path for each group so as to form the array; and

in response to detection of an error in dispensers of different columns within first and second rows of a first group;

(i) positions the head with a first row of each group aligned with the selected path for that group;

(ii) moves the head with respect to the substrate while dispensing droplets from said second dispenser in the first row of the first group in accordance with a part of the pattern for the selected path for that group;

(iii) re-positions the head such that a second row of each group is aligned with the selected paths; and

(iv) moves the head with respect to the substrate while dispensing droplets from non-error dispensers in the second row of the first group in accordance with a part of the pattern for that group.

12. An apparatus according to claim 11 wherein the dispensers are pulse jets.

13. An apparatus according to claim 11 wherein in step (iv) droplets are dispensed from non-error dispensers in the second row of each of multiple groups in accordance with at least part of the pattern for each group.

14. An apparatus according to claim 11 wherein:

said dispensers of a same column within a group are loaded with a same fluid by communicating with a corresponding common reservoir, and in step (iv) droplets are dispensed from a non-error dispenser that is in the same column as the error dispenser.

15. An apparatus according to claim 11 wherein in step (iv) droplets are dispensed from at least one second row of a group in the complete pattern for the selected path of the same group.

16. An apparatus according to claim 11 additionally comprising a sensor to monitor dispensers for an error and provide corresponding data to the processor.

17. A method of fabricating a chemical array using:

a head system with multiple groups of drop dispensers which move in unison, wherein each group comprises a series of drop dispensers, and wherein said series comprises at least a first and a second drop dispenser loaded with a same fluid;

a transport system to move the head system with respect to a substrate with different dispensers in the series of each group following respective paths;

a processor to dispense droplets from a single dispenser of each group in a pattern along a selected path for each group during operation of the transport system, so as to form the array;

the method comprising;

a) identifying an error in said first drop dispenser of a group; and

b) moving said group of dispensers to dispense droplets from said second drop dispenser of said group along at least part of the path selected for said group.

18. A method according to claim 17 wherein in step (b) droplets are dispensed from each second dispenser of the series within each of multiple groups in at least part of the pattern for the selected path of the group containing that series.

19. A method according to claim 18 wherein:

a series of dispensers within a group communicate with a corresponding common reservoir for that series.

20. A method according to claim 18 wherein in step (b) the droplets are dispensed from a second dispenser of a series in a complete pattern for the first dispenser of the series containing the second dispenser.

21. A method according to claim 18 wherein in step (b) the droplets are dispensed from at least one second dispenser of a first group, in a complete pattern for the selected path of the first group.

22. A method according to claim 18 wherein when a second dispenser of a second group is additionally in error, the first and second dispensers of each group are alternately moved along the selected path for that group while droplets are dispensed from non-error dispensers of the first and second groups in at least part of the pattern for the selected paths for the first and second groups.

23. A method according to claim 17 wherein the dispensers are pulse jets.

24. A method according to claim 17 wherein the array comprises biopolymer features.

25. A method according to claim 24 wherein the array comprises peptide containing features.

26. A method according to claim 17 wherein the array carries polynucleotide containing features.

27. A method according to claim 17 wherein the array carries features with polynucleotides of different sequence.

28. A method of fabricating a chemical array using:

a head system with multiple groups of drop dispensers which move in unison, wherein each group comprises multiple rows and columns of dispensers and wherein at least two dispensers of a same column within a group are loaded with a same fluid;

a transport system to move the head system with respect to a substrate with different rows following respective paths;

a processor to dispense droplets from dispensers during operation of the transport system, in a pattern along a selected path for each group, so as to form the array;

the method comprising;

a) identifying an error in one or more dispensers; and

b) when in response to identifying an error in one or more dispensers of different columns within first and second rows of a first group;

(i) positioning the head with a first row of each group aligned with the selected path for that group;

(ii) moving the head with respect to the substrate while dispensing droplets from non-error dispensers in the first row of the first group in accordance with a part of the pattern for the selected path for that group;

(iii) re-positioning the head such that a second row of each group is aligned with the selected paths; and

(iv) moving the head with respect to the substrate while dispensing droplets from non-error dispensers in the second row of the first group in accordance with a part of the pattern for that group.

29. A method according to claim 28 wherein the dispensers are pulse jets.

30. A method according to claim 28 wherein in step (b)(iv) droplets are dispensed from non-error dispensers in the second row of each of multiple groups in accordance with at least part of the pattern for each group.

31. A method according to claim 28 wherein:

said dispensers of a same column within a group are loaded with a same fluid by communicating with a corresponding common reservoir; and

in step (b)(iv) droplets are dispensed from a non-error dispenser in the same column and group as the error dispenser.

32. A method according to claim 28 wherein in step (b)(iv) droplets are dispensed from at least one second row of a group in the complete pattern for the selected path of the group containing that second row.