Encoding and decoding of array sensors utilizing nanocrystals

• US 6,890,764 B2
• Filed: 12/31/2002
• Issued: 05/10/2005
• Est. Priority Date: 05/20/1999
• Status: Expired due to Term

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Zhao, Chanfeng, Chee, Mark S., Barnard, Steven M.
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): Do, Pensee T.
• Application Number:
  US10/334,416
• Publication Number:
  US 20030175773A1
• Time in Patent Office: 861 Days
• Field of Search: 436/518, 436/5, 436/62, 436/4, 436/523, 436/524, 436/164, 436/805, 436/823, 435/7.1, 435/174
• US Class Current: 436/518
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/587 Nanoparticles
• CPC Class Codes: G01N 33/588 with semiconductor nanocrystal label, e.g. quantum dots
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/823 Immunogenic carrier or carrier per se
• CPC Class Codes: Y10S 977/70 Nanostructure
• CPC Class Codes: Y10S 977/776 Ceramic powder or flake
• CPC Class Codes: Y10S 977/789 in array format
• CPC Class Codes: Y10S 977/792 Nucleic acid array, e.g. human genome array
• CPC Class Codes: Y10S 977/795 Composed of biological material
• CPC Class Codes: Y10S 977/898 Enzymatic
• CPC Class Codes: Y10S 977/902 Specified use of nanostructure
• CPC Class Codes: Y10S 977/904 for medical, immunological, body treatment, or diagnosis
• CPC Class Codes: Y10S 977/915 Therapeutic or pharmaceutical composition

Abstract

Described herein are assays and components for encoding and decoding microspheres. Each assay or component described utilizes at least one nanocrystal.

Claims

1. A method of identifying the location of at least a plurality of a bioactive agent, comprising

a) providing an array composition comprising:

i) a substrate with a surface comprising discrete sites wherein said discrete site are wells; and

ii) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises said bioactive agent;

wherein said microspheres are distributed at said;

b) adding a plurality of decoding binding ligands comprising optical signatures to said array composition to identify the location of at least said plurality of the bioactive agents, wherein at least one of said optical signatures comprises a nanocrystal.

2. A method according to claim 1 wherein at least one subpopulation of microspheres comprises an identifier binding ligand to which a decoding binding ligand can bind.

3. A method according to claim 1 wherein said decoding binding ligands bind to said bioactive agents.

4. A method according to claim 1 wherein the location of each subpopulation is determined.

5. The method according to claim 1, 2, 3 or 4, wherein said substrate is selected from the group consisting of glass, plastic and fiber optic bundles.

6. The method according to claim 5, wherein said substrate is glass.

7. The method according to claim 5, wherein said substrate is plastic.

8. The method according to claim 5, wherein said substrate is a fiber optic bundle.

9. The method according to claim 1, 2, 3 or 4, wherein at least one of said bioactive agents is a nucleic acid.

10. The method according to claim 1, 2, 3 or 4, wherein at least one of said bioactive agents a protein.

11. The method according to claim 2, wherein at least one of said identifier binding ligands is a nucleic acid and at least one of said decoder binding ligands is a nucleic acid.

12. The method according to claim 2, wherein at least one of said identifier binding ligands is a protein and at least one of said decoder binding ligands is an antibody.

13. The method according to claim 3, wherein at least one of said bioactive agents is a nucleic acid and at least one decoder binding ligand is a nucleic acid.

14. The method according to claim 3, wherein at least one of said bioactive agents is a protein and at least one of said decoder binding ligands is an antibody.

15. A method of identifying the location of at least a plurality of a bioactive agent, comprising:

contacting an array composition comprising;

a) a substrate with a surface comprising discrete sites, wherein said discrete sites are wells; and

b) a population of microspheres comprising at least a first and second subpopulation, wherein each subpopulation comprises said bioactive agent, wherein said microspheres are distributed at said sites

with a plurality of decoding binding ligands comprising optical signatures to identify the location of at least said plurality of the bioactive agents, wherein at least one of said optical signatures comprises a nanocrystal.