Microsensors for glucose and insulin monitoring
First Claim
Patent Images
1. An apparatus for producing a signal related to the concentrations of glucose and insulin, said apparatus comprising:
- a first conductive composite comprising a glucose oxidase and a metal posited within a first cylindrical tube;
a second conductive composite comprising a RuOx or IrOx catalyst posited within a second cylindrical tube;
a sleeve containing the first cylindrical tube and the second cylindrical tube;
a first electrical contact within the first cylindrical tube in contact with the first conductive composite; and
a second electrical contact within the second cylindrical tube in contact with the second conductive composite.
2 Assignments
0 Petitions
Accused Products
Abstract
A dual sensor for the simultaneous amperometric monitoring of glucose and insulin, wherein the glucose probe is based on the biocatalytic action of glucose oxidase, and the insulin probe is based on the electrocatalytic activity of metal oxide. Further provided is an oxidase enzyme composite electrode with an internal oxygen-rich binder. The present invention also optionally includes metallizing components within the carbon paste to eliminate signals from interfering compounds. The present invention includes embodiments for both in vitro and in vivo uses.
514 Citations
46 Claims
-
1. An apparatus for producing a signal related to the concentrations of glucose and insulin, said apparatus comprising:
-
a first conductive composite comprising a glucose oxidase and a metal posited within a first cylindrical tube;
a second conductive composite comprising a RuOx or IrOx catalyst posited within a second cylindrical tube;
a sleeve containing the first cylindrical tube and the second cylindrical tube;
a first electrical contact within the first cylindrical tube in contact with the first conductive composite; and
a second electrical contact within the second cylindrical tube in contact with the second conductive composite. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. An apparatus for producing a signal related to the concentration of a substance, said apparatus comprising:
-
a first conductive composite comprising an oxygen-dependent enzyme and sufficient oxygen-rich binder to support an oxygen-dependent enzymatic reaction in the absence of exogenous oxygen, a second conductive composite comprising a RUOx or IrOx catalyst;
a sleeve with a first end and second end;
a first cavity disposed within the first end of said sleeve wherein the length of the sleeve is at least about 0.2 mm, said cavity containing said first composite and forming an electrode end of said composite at the first end of said sleeve;
a second cavity containing said second composite; and
an electrical contact disposed within the second end of said sleeve extending into said cavity;
wherein the ratio of the surface area of the electrode end of said composite to the volume of said composite is at least about 1;
8. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
-
27. A method of simultaneous detection of glucose and insulin in a liquid sample, the method comprising the following steps:
-
a) providing a first screen-printed, thick-film biosensor comprising glucose oxidase;
b) providing a second screen-printed, thick-film biosensor comprising a metal oxide catalyst;
c) simultaneously placing the first biosensor and the second biosensor in the liquid sample;
d) applying a detection potential to the first biosensor and the second biosensor;
e) detecting a biocatalytic signal at the first biosensor; and
f) detecting an electrocatalytic signal at the second biosensor wherein providing a first screen-printed, thick-film biosensor comprising glucose and providing a second screen-printed, thick-film biosensor comprising a metal oxide catalyst comprises providing a dual sensor test strip with the first screen-printed, thick-film biosensor and second screen-printed, thick-film biosensor disposed thereon. - View Dependent Claims (28, 29, 30, 31)
-
-
32. A method of detecting substrate concentration of an environment using a composite biosensor, the method comprising the following steps:
-
a) providing a biosensor comprising (1) a first conductive composite comprising sufficient oxygen-rich binder to support an oxygen-dependent enzymatic reaction in the absence of exogenous oxygen, a conductor, an oxygen-dependent enzyme, a first reservoir containing the first composite, and an electrode end of the first composite, wherein the ratio of the surface area of the electrode end of said first composite to the volume of said first composite is at least about 1;
8, and (2) a second reservoir containing a second conductive composite comprising a metal oxide selected from the group consisting of RuOx and IrOx,b) placing the biosensor in the environment, and c) detecting the substrate concentration. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
-
Specification