Gene detection system, gene detection device comprising same, detection method, and gene detecting chip

• US 6,893,824 B2
• Filed: 01/18/2002
• Issued: 05/17/2005
• Est. Priority Date: 01/19/2001
• Status: Expired due to Fees

• Current Assignee: Toppan Printing Co., Ltd. (TOPPAN Holdings, Inc.)
• Original Assignee: SII Nano Technology Inc. (Hitachi, Ltd.), Tum Gene, Inc.
• Inventors: Ito, Tetsumasa
• Primary Examiner(s): Forman, BJ
• Application Number:
  US10/050,608
• Publication Number:
  US 20020155477A1
• Time in Patent Office: 1,215 Days
• Field of Search: 435/6, 435/174, 435/283.1, 435/287.2, 435/287.3, 536/24.3, 536/23.1, 422/68.1, 422/82.01
• US Class Current: 435/6.11
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A gene detection system for detecting a target gene upon hybridization with a probe comprising a probe-immobilizing support on which a probe is immobilized, and heating and cooling means disposed in contact with another location different from the surface of the probe-immobilizing support on which the probe is immobilized, whereby genes can be rapidly detected with high sensitivity through hybridization.

Claims

1. A gene detection system for detecting a target gene upon hybridization with a probe, said gene detection system comprising:

an electrode substrate having a first surface and a second surface, the first surface being opposite to the second surface through the electrode substrate;

an electrode provided on the first surface, the electrode having a probe immobilizing surface;

a soaking component provided on the second surface;

heating and cooling means provided on the soaking component; and

a heat insulating member covering a circumferential surface of the electrode,

wherein a thermal capacity of the soaking component is greater than that of the electrode substrate.

2. The gene detection system according to claim 1, wherein the soaking component comprises a temperature sensor for measuring a temperature of the soaking component.

3. The gene detection system according to claim 1, wherein the heating and cooling means comprises a Peltier element or a heater.

4. The gene detection system according to claim 1, wherein the heating and cooling means comprises a heat sink at an opposite side from the soaking component.

5. The gene detection system according to claim 1, wherein at least a portion of a surface of the electrode comprises gold, silver, or copper, and the electrode substrate comprises a ceramic material.

6. The gene detection system according to claim 1, wherein the electrode comprises a plurality of electrodes.

7. The gene detection system according to claim 6, wherein each of a plurality of probes is immobilized on each of the plurality of electrodes, and wherein each of the plurality of probes detects a predetermined base sequence different from each of the other plurality of probes.

8. The gene detection system according to claim 6, wherein the heating and cooling means independently controls a temperature of each of the plurality of electrodes.

9. The gene detection system according to claim 7, wherein the heating and cooling means independently controls a temperature of each of the plurality of electrodes.

10. The gene detection system according to claim 1, wherein the electrode forms a pin.

11. The gene detection system according to claim 1, wherein the heat insulating member comprises polyether ether ketone or polytetrafluoroethylene.

12. A gene detection device, comprising:

the gene detection system according to any one of claims 1-11; and

control means for controlling an actuation of the heating and cooling means.

13. A gene detection system comprising:

an electrode substrate having a first surface and a second surface, the first surface being opposite to the second surface through the electrode substrate;

a cylindrical electrode projecting from the first surface of the electrode substrate, wherein a probe-immobilizing surface of the cylindrical electrode is disposed away from the electrode substrate, and wherein an insulating member substantially covers a circumferential surface of the cylindrical electrode;

a soaking component provided on the second surface; and

heating and cooling means disposed in contact with the soaking component,

wherein a thermal capacity of the soaking component is greater than that of the electrode substrate.

14. The gene detection system according to claim 13, wherein the soaking component comprises a temperature sensor for measuring a temperature of the soaking component.

15. The gene detection system according to claim 13, wherein the heating and cooling means comprises a Peltier element or a heater.

16. The gene detection system according to claim 13, wherein the heating and cooling means comprises a heat sink at an opposite side from the soaking component.

17. The gene detection system according to claim 13, wherein at least a portion of a surface of the cylindrical electrode comprises gold, silver, or copper, and the electrode substrate comprises a ceramic material.

18. The gene detection system according to claim 13, wherein the cylindrical electrode comprises a plurality of cylindrical electrodes.

19. The gene detection system according to claim 18, wherein each of a plurality of probes is immobilized on each of the plurality of cylindrical electrodes, and wherein each of the plurality of probes detects a predetermined base sequence different from each of the other plurality of probes.

20. The gene detection system according to claim 18, wherein the heating and cooling means independently controls a temperature of each of the plurality of cylindrical electrodes.

21. The gene detection system according to claim 19, wherein the heating and cooling means independently controls a temperature of each of the plurality of cylindrical electrodes.

22. The gene detection system according to claim 13, wherein the cylindrical electrode forms a pin.

23. The gene detection system according to claim 13, wherein the heat insulating member comprises polyether ether ketone or polytetratluoroethylene.

24. A gene detection device, comprising:

the gene detection system according to any one of claims 13-23; and

control means for controlling an actuation of the heating and cooling means.