PCR thermocycler

• US 6,893,863 B2
• Filed: 09/27/2002
• Issued: 05/17/2005
• Est. Priority Date: 11/25/1998
• Status: Expired due to Fees

• Current Assignee: Lawrence Livermore National Security LLC (Government of the United States of America)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Benett, William J., Richards, James B.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US10/260,120
• Publication Number:
  US 20030036189A1
• Time in Patent Office: 963 Days
• Field of Search: 422/102, 422/129, 422/240, 422/241, 422/131, 422/82.01, 435/285.1, 435/287.2, 435/303.1, 935/88
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00788 Three-dimensional assemblies, i.e. the reactor comprising a form other than a stack of plates
• CPC Class Codes: B01J 2219/0081 Plurality of modules
• CPC Class Codes: B01J 2219/00828 Silicon wafers or plates
• CPC Class Codes: B01J 2219/00873 Heat exchange
• CPC Class Codes: B01J 2219/00961 Temperature
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1838 using fluid heat transfer medium
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A sleeve-type silicon polymerase chain reaction (PCR) chamber or thermocycler having improved thermal performance. The silicon sleeve reaction chamber is improved in thermal performance by etched features therein that reduce thermal mass and increase the surface area of the sleeve for cooling. This improved thermal performance of the thermocycler enables an increase in speed and efficiency of the reaction chamber. The improvement is accomplished by providing grooves in the faces of the sleeve and a series of grooves on the interior surfaces that connect with grooves on the faces of the sleeve. The grooves can be anisotropically etched in the silicon sleeve simultaneously with formation of the chamber.

Claims

1. A microfabricated chemical reactor apparatus, comprising:

a sleeve that forms a reaction chamber, said sleeve having at least one external surface and at least one internal surface,

a plurality of grooves in said sleeve, said plurality of groves including

at least one longitudinally extending groove on said at least one external surface of said sleeve and

at least one radially extending groove on said at least one internal surface of said sleeve,

wherein said at least one longitudinally extending groove and said at least one longitudinally extending groove reduces thermal mass and increases surface area of said sleeve reaction chamber.

2. The microfabricated chemical reactor apparatus of claim 1, wherein said at least one longitudinally extending groove, and said at least one radially extending groove are connected to form a passageway there-through.

3. The microfabricated chemical reactor apparatus of claim 1, wherein said plurality of grooves includes at least one longitudinally extending groove on opposite sides of said sleeve, and at least one radially extending groove connected to each of said longitudinally extending grooves to form passageways there-through.

4. The microfabricated chemical reactor apparatus of claim 3, wherein two longitudinally extending grooves are located on opposite sides of said sleeve, and wherein said plurality of connecting radially extending grooves are located along opposite end sections of said sleeve.

5. The microfabricated chemical reactor apparatus of claim 4, wherein each of said grooves is of a V-shaped configuration.

6. The microfabricated chemical reactor apparatus of claim 5, wherein said sleeve is constructed of silicon.

7. The microfabricated chemical reactor apparatus of claim 1, wherein said sleeve reaction chamber is composed of two sections, said sections being bonded or clamped together.

8. The microfabricated chemical reactor apparatus of claim 7, wherein said two sections are configured to define a chamber to enclose an insert therebetween such that said insert is in direct contact with at least a portion of walls of said two sections defining the chamber or spaced from the walls of said two sections defining the chamber.

9. A microfabricated chemical reactor apparatus, comprising:

an elongated sleeve that forms a silicon-based sleeve reaction chamber, said elongated silicon-based sleeve reaction chamber

having at least one external surface and

having at least one internal surface,

a plurality of longitudinally extending grooves on said at least one external surface of said silicon-based elongated sleeve reaction chamber and

a plurality of radially extending grooves on said at least one internal surface of said elongated silicon-based sleeve reaction chamber,

wherein said plurality of longitudinally extending grooves on said at least one external surface of said elongated silicon-based sleeve reaction chamber and wherein said a plurality of radially extending grooves on said at least one internal surface of said elongated silicon-based sleeve reaction chamber reduces thermal mass and also increases cooling surface area of said elongated silicon-based sleeve reaction chamber.

10. The microfabricated chemical reactor apparatus of claim 9, wherein said plurality of grooves include interconnected sections to define passageways in said sleeve.

11. The microfabricated chemical reactor apparatus of claim 10, wherein said plurality of grooves include at least one longitudinally extending groove and at least one radially extending groove connected to said at least one longitudinally extending groove.

12. The microfabricated chemical reactor apparatus of claim 11, wherein at least one longitudinally extending groove is located on opposite sides of said sleeve.

13. The microfabricated chemical reactor apparatus of claim 12, wherein at least one radially extending groove is located in opposite end sections of said sleeve.

14. The microfabricated chemical reactor apparatus of claim 13, wherein two longitudinally extending grooves are located in spaced relation on each side of said sleeve.

15. The microfabricated chemical reactor apparatus of claim 9, wherein said sleeve is composed of two body sections, and wherein said slot is formed by a cutaway in each of said body sections, and wherein the microfabricated chemical reactor apparatus comprises said plurality of longitudinally and radially extending grooves being located in each of said body sections.

16. The microfabricated chemical reactor apparatus of claim 15, wherein said plurality of grooves includes at least one longitudinally extending groove in a surface opposite said cutaway and in spaced relation from said cutaway, and includes a series of radially extending grooves in the same surface as said cutaway and in spaced relation to said cutaway.

17. The microfabricated chemical reactor apparatus of claim 9, including a heater and temperature sensors formed of a platinum film.