Analyte assays and devices
First Claim
1. A device for performing a binding pair assay comprising:
- (a) a housing comprising a sampler member and a detection member, wherein the device is capable of being separated into at least two parts comprising the sampler member and the detection member, and the device is further capable of being assembled into one part;
(b) a chromatography region associated with the detection member comprising a chromatography medium having a transit zone and a capture zone;
(c) a sample collector associated with the sampler member that is exposed to receive sample upon separation of the device into the sampler member and the detection member, and wherein the sample collector is in capillary communicating contact with the capture zone through the transit zone when the device is assembled and;
(d) a reagent delivery system positioned relative to the sampler member for delivering a liquid reagent to the sample collector, wherein the reagent delivery system is positioned so that the liquid regent is added to the reagent delivery system after the sampler member and detection member are assembled into one part and flows through the sample collector and into the chromatography region.
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Abstract
The present invention provides a method and a device that utilizes capillarity-mediated, chromatographic transport, for the qualitative or semi-quantitative analysis of selected analytes in liquid samples. The device utilizes an applicator/collection device for collecting and administering the sample to the flow path such that reagent(s) flow through the applicator/collection device, washing the sample into the reaction pathway. The device further utilizes an air gap between the initial location of the reagent and the reaction pathway to funnel the reagent efficiently through the sample so as to collect all or substantially all of the sample and make it available for the reaction(s).
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Citations
33 Claims
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1. A device for performing a binding pair assay comprising:
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(a) a housing comprising a sampler member and a detection member, wherein the device is capable of being separated into at least two parts comprising the sampler member and the detection member, and the device is further capable of being assembled into one part;
(b) a chromatography region associated with the detection member comprising a chromatography medium having a transit zone and a capture zone;
(c) a sample collector associated with the sampler member that is exposed to receive sample upon separation of the device into the sampler member and the detection member, and wherein the sample collector is in capillary communicating contact with the capture zone through the transit zone when the device is assembled and;
(d) a reagent delivery system positioned relative to the sampler member for delivering a liquid reagent to the sample collector, wherein the reagent delivery system is positioned so that the liquid regent is added to the reagent delivery system after the sampler member and detection member are assembled into one part and flows through the sample collector and into the chromatography region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification