Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices

• US 6,898,458 B2
• Filed: 12/19/2001
• Issued: 05/24/2005
• Est. Priority Date: 12/19/2000
• Status: Expired due to Fees

• Current Assignee: Verisante Technology Inc.
• Original Assignee: Perceptronix Medical Inc.
• Inventors: Zeng, Haishan, Lam, Stephen, Palcic, Branko Michael
• Primary Examiner(s): ROBINSON, DANIEL LEON
• Application Number:
  US10/028,568
• Publication Number:
  US 20020103439A1
• Time in Patent Office: 1,252 Days
• Field of Search: 600/476, 600/407, 600/473, 600/322, 600/162, 600/477, 600/478, 600/479, 600/480, 250/317.1, 250/318, 350/451
• US Class Current: 600/476
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/021 using plane or convex mirrors, parallel phase plates, or particular reflectors
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0235 using means for replacing an element by another, for replacing a filter or a grating
• CPC Class Codes: G01J 3/0243 having a through-hole enabling the optical element to fulfil an additional optical function, e.g. a mirror or grating having a throughhole for a light collecting or light injecting optical fiber
• CPC Class Codes: G01J 3/0289 Field-of-view determination; Aiming or pointing of a spectrometer; Adjusting alignment; Encoding angular position; Size of measurement area; Position tracking
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/36 Investigating two or more bands of a spectrum by separate detectors
• CPC Class Codes: G01N 2223/076 X-ray fluorescence
• CPC Class Codes: G01N 23/223 by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence

Abstract

Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Claims

1. A light beam detection system comprising:

an area sized to receive the light beam;

a beam separator disposed in the area to separate a small portion of the light beam from a remainder of the light beam to provide a separated light beam and a remainder light beam and to transmit the separated light beam to a spectroscopy device;

an imaging device disposed in the area to operably receive the remainder light beam to provide an image therefrom; and

,

a spectroscopy device optically connected to the beam separator to receive the separated light beam to provide a spectrum therefrom, wherein the beam separator is located substantially in front of and does not touch the imaging device and wherein the system further comprises a first focusing element in front of the beam separator and a second focusing element between the beam separator and the imaging device, the first focusing element and the second focusing element located to provide a first conjugate image plane substantially at the beam separator and a second conjugate image plane substantially at the imaging device.

2. The light beam detection system of claim 1 wherein the beam separator abuts the imaging device.

3. A light beam detection system comprising:

an area sized to receive the light beam;

a beam separator disposed in the area to separate a small portion of the light beam from a remainder of the light beam to provide a separated light beam and a remainder light beam and to transmit the separated light beam to a spectroscopy device;

an imaging device disposed in the area to operably receive the remainder light beam to provide an image therefrom; and

,

a spectroscopy device optically connected to the beam separator to receive the separated light beam to provide a spectrum therefrom, wherein the system further comprises an image display device operably connected to the imaging device to display an image from the imaging device and a spectrum display device operably connected to the spectroscopy device to display a spectrum from the spectroscopy device, and wherein the image display device and the spectrum display device is a single display device operable to contemporaneously display both the image from the imaging device and the spectrum from the spectroscopy device.

4. A light beam detection system comprising:

an area sized to receive the light beam;

a beam separator disposed in the area to separate a small portion of the light beam from a remainder of the light beam to provide a separated light beam and a remainder light beam and to transmit the separated light beam to a spectroscopy device;

an imaging device disposed in the area to operably receive the remainder light beam to provide an image therefrom; and

,

a spectroscopy device optically connected to the beam separator to receive the separated light beam to provide a spectrum therefrom, wherein the system further comprises an image display device operably connected to the imaging device to display an image from the imaging device and a spectrum display device operably connected to the spectroscopy device to display a spectrum from the spectroscopy device, and wherein the system further comprises an image separator comprising a plurality of light selection elements that separates the image into a plurality of selected wavelength region images wherein each selected wavelength region image corresponds to a different wavelength region of the range of wavelengths in the light beam, wherein the image separator comprises a plurality of imaging beam splitters each of which selects for different selected wavelength regions to provide the selected wavelength region images and directs the selected wavelength region images to different imaging devices.

5. The light beam detection system of claim 4 wherein the different imaging devices are different regions of a single imaging detector.

6. The light beam detection system of claim 4 wherein the imaging beam splitters are disposed linearly along the light beam.

7. The light beam detection system of claim 4 wherein there are at least three imaging beam splitters disposed to reflect the selected wavelength region images in at least three different radial directions and the different imaging devices are disposed radially about the light beam to receive the selected different wavelength images.

8. The light beam detection system of claim 4 wherein the imaging beam splitters are disposed alternatingly such that a first sub-set of the imaging beam splitters direct a first set of selected wavelength region images in a first direction and a second sub-set of the imaging beam splitters direct a second set of selected wavelength region images in a second direction that is substantially 90°

or 180°

away from the first direction.

9. The light beam detection system of claim 4 wherein the imaging beam splitters select for all but one desired, non-selected different wavelength region to provide a non-selected wavelength region image, the image separator further comprising an imaging device located in the light beam and behind the imaging beam splitters to directly receive the non-selected wavelength region image.

10. The light beam detection system of claim 4 wherein the different wavelength regions comprise UV to blue light, visible light, near-infrared light and infrared light.

11. The light beam detection system of claim 10 wherein the display device is operably connected to the image separator to contemporaneously display at least two images selected from the UV to blue light, visible light, near-infrared light and infrared light, and wherein the display device is further able to contemporaneously display the spectrum from the spectroscopy device.