Automated sample treatment system: apparatus and method

• US 6,899,848 B1
• Filed: 02/27/2001
• Issued: 05/31/2005
• Est. Priority Date: 02/27/2001
• Status: Active Grant

• Current Assignee: Hamilton Company
• Original Assignee: Hamilton Company
• Inventors: Kelley, Sean Patrick, Chen, Chih-Chung, Gulla, Gregory
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R.
• Application Number:
  US09/795,853
• Time in Patent Office: 1,554 Days
• Field of Search: 422 63- 67, 422/68.1, 422/100, 422/99, 422/101
• US Class Current: 422/63
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom

Abstract

A sample treatment system for automated extraction of compounds is disclosed. The system includes a housing defining an enclosure including a top cover having open ended chambers to receive sample plates on an interposed carriage frame, an automated shuttle means for lateral transportation and vertical lifting of the carriage frame to selectively address the chamber below the sample plate for the throughpassage of fluid from the sample plate and urged by a vacuum pump for evacuating air from a chamber to a degree below atmospheric pressure for through passage of fluid from the sample plate to either a waste plate or a collection plate. The shuttle means includes motor drives for precisely laterally and vertically positioning the carriage frame.

Claims

1. A sample treatment device, comprising, in combination:

a housing having plural chambers, one of said chambers including means for directing waste fluid from said chamber to a waste storage area, said waste storage area operatively coupled to said one said chamber by means of a pump;

a sample plate;

means to expose said sample plate to one chamber in said housing;

extraction means operating on the sample plate addressing the sample plate to the one chamber, said sample plate in fluid communication with said one chamber, whereby said extraction means extract components of interest from samples residing in said sample plate; and

shuttle means to selectively present the sample plate to one of the plural chambers for the throughpassage of fluid from the sample plate, said shuttle means including;

a carriage frame upon which said sample plate is placed,

lifting means for elevating said carriage frame from a first elevation to a second elevation, and

means for translating said carriage frame laterally along a substantially horizontal plane, wherein said lateral translation means includes a belt driven trolley which moves in response to motion of said belt.

2. The device of claim 1 wherein said means to expose said sample plate to one chamber in said housing includes an open top and bottom of said carriage frame, said carriage frame formed from a rectangular construct having four sides.

3. The device of claim 2 wherein said lifting means includes two pairs of lift rods extending between said carriage frame and two pairs of cam links, said cam links connected to a pair of shafts, two links per said shaft and said cam links driven by a pair of meshing gears, one said gear mounted per shaft, and one said shaft driven by a motor.

4. A sample treatment device, comprising, in combination:

a housing having plural chambers, one of said plural chambers including a positioner located on a floor of one said chamber, said positioner including a marginal portion which projects upwardly from said positioner to support a collection plate thereupon;

a sample plate;

means to expose said sample plate to one chamber in said housing;

extraction means operating on the sample plate addressing the sample plate to the one chamber, said sample plate in fluid communication with said one chamber, whereby said extraction means extract components of interest from samples residing in said sample plate; and

shuttle means to selectively present the sample plate to one of the plural chambers for the throughpassage of fluid from the sample plate.

5. A sample treatment device, comprising, in combination:

a housing having plural chambers, one of said plural chambers including a waste container resting on a floor of said one chamber having an isolation plate resting on said waste container, said waste container and said one chamber provided with means for removing waste fluid therefrom;

a sample plate;

means to expose said sample plate to one chamber in said housing;

extraction means operating on the sample plate addressing the sample plate to the one chamber, said sample plate in fluid communication with said one chamber, whereby said extraction means extract components of interest from samples residing in said sample plate; and

shuttle means to selectively present the sample plate to one of the plural chambers for the throughpassage of fluid from the sample plate.

6. The device of claim 5 wherein said sample plate has open ends and said isolation plate includes a downwardly extending peripheral skirt dimensioned to depend within said waste container from a shelf located on said isolation plate, and said isolation plate includes a plurality of wells passing through said isolation plate having a dimension such that when said open ends on said sample plate project within said wells, each said open end is protected from cross-talk with adjacent said open ends.