Methods of monitoring effects of chemical agents on a sample
First Claim
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1. A method for monitoring effects of a chemical agent on a sample, the method comprising the steps of:
- dispensing a chemical agent on a sample;
providing an automated triggering signal to initiate a measurement period relative to said dispensing step;
capturing a plurality of sequential images of said sample during said measurement period;
automatically aligning a subset of said plurality of images to compensate for sample motion; and
measuring an optical signal observed from said sample within said measurement period.
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Abstract
The invention provides methods and systems for monitoring effects of chemical agents on optical signals produced by samples in response to the chemical agents. Preferred methods comprise application of multiple chemical agents that interact to alter an optical signal from the sample. Methods and systems of the invention also comprise monitoring an optical signal from an endogenous chromophore upon application of a chemical agent to a sample. Methods and systems of the invention also comprise the use of triggers, atomizers and image alignment to enhance the results of methods described herein.
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Citations
9 Claims
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1. A method for monitoring effects of a chemical agent on a sample, the method comprising the steps of:
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dispensing a chemical agent on a sample;
providing an automated triggering signal to initiate a measurement period relative to said dispensing step;
capturing a plurality of sequential images of said sample during said measurement period;
automatically aligning a subset of said plurality of images to compensate for sample motion; and
measuring an optical signal observed from said sample within said measurement period. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification