Irreversible immobilization of enzymes into polyurethane coatings
First Claim
1. A method of irreversibly immobilizing enzymes into polyurethane coatings comprising the steps of:
- reacting a mixture of a polyol dispersion coreactant, and an enzyme to create an aqueous mixture;
adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating; and
curing the enzyme-containing polyurethane coating.
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Abstract
This invention provides a method of irreversibly immobilizing an enzyme into polyurethane coatings. This invention also provides for an enzyme-containing coating having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethane coatings in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the coating. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.
152 Citations
20 Claims
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1. A method of irreversibly immobilizing enzymes into polyurethane coatings comprising the steps of:
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reacting a mixture of a polyol dispersion coreactant, and an enzyme to create an aqueous mixture;
adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating; and
curing the enzyme-containing polyurethane coating. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of irreversibly immobilizing diisopropylfluorophosphatase into polyurethane coatings comprising the steps of:
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reacting a mixture of a polyol dispersion coreactant having a water content of 70 w. %, a polyether modified polydimethyl siloxane surfactant, a buffered medium comprised of bis-tris-propane buffer and CaCl2 and diisopropylfluorophosphatase to create an aqueous mixture;
adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating; and
curing the enzyme-containing polyurethane coating. - View Dependent Claims (10, 11)
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12. An enzyme-containing composition made by the process comprising the steps of:
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reacting a mixture of a polyol dispersion coreactant and an enzyme to create an aqueous mixture;
adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating; and
curing the enzyme-containing polyurethane coating. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
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Specification