Device for placement of effluent

• US 6,911,182 B2
• Filed: 10/18/2002
• Issued: 06/28/2005
• Est. Priority Date: 10/18/2002
• Status: Expired due to Fees

• Current Assignee: Indiana University Research & Technology Corporation (Indiana University)
• Original Assignee: Indiana University Research & Technology Corporation (Indiana University)
• Inventors: Novotny, Milos V., Reilly, James P., Boraas, Kirk S., Mechref, Yehia, Tegeler, Tony J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Sines, Brian
• Application Number:
  US10/274,247
• Publication Number:
  US 20040076549A1
• Time in Patent Office: 984 Days
• Field of Search: 422/50, 422/63, 422/64, 422/56, 422/66, 422/67, 422/68.1, 422/70, 422/81, 422/82, 422/99, 422/100, 422/101, 422/103, 422/65, 436/43, 436/44, 436/46, 436/47, 436/180, 436/174, 435/283.1, 435/286.4, 435/287.1, 435/287.3
• US Class Current: 422/509
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: G01N 2030/8417 the store moving as a whole, e.g. moving wire
• CPC Class Codes: G01N 30/84 Preparation of the fraction to be distributed
• CPC Class Codes: H01J 49/00 Particle spectrometers or separator tubes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/110833 Utilizing a moving indicator strip or tape
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A device for placement of effluent comprises a substrate positioner, a deposition conduit, and a conduit positioner. The substrate positioner supports and positions a substrate on which effluent exiting from the deposition conduit is to be deposited. The conduit positioner moves an exit end of the deposition conduit relative to the substrate.

Claims

1. A device for placement of effluent on a substrate, the device comprising

a substrate positioner configured to support and position the substrate,

a deposition conduit having a first opening through which effluent enters the conduit and having an exit end with a second opening through which effluent exits the conduit, and

a conduit positioner configured to move the exit end of the deposition conduit between a raised position in which surface tension of the effluent adjacent the second opening prevents the effluent from separating from the exit end of the deposition conduit and a lowered position in close proximity to the substrate so that effluent adjacent the second opening is transferred from the conduit to the substrate as a result of contacting and adhering to the substrate.

2. The device of claim 1, wherein the substrate positioner comprises an X-Y table that is operable to move the substrate in an x-direction and a y-direction.

3. The device of claim 1, wherein the substrate positioner comprises a reel-to-reel tape drive that is operable to move the substrate from a first reel to a second reel.

4. The device of claim 1, further comprising a fluid treatment device coupled to the deposition conduit.

5. The device of claim 4, wherein the fluid treatment device operates to output the effluent into the deposition conduit in a form suitable for matrix-assisted laser desorption ionization.

6. The device of claim 1, wherein the conduit positioner comprises an arm, the deposition conduit is coupled to the arm, and the arm is movable to move the exit end of the deposition conduit between the raised position and the lowered position.

7. The device of claim 6, wherein the conduit positioner further comprises a motor having an output shaft and a cam coupled to the output shaft, the arm engages the cam, and rotation of the output shaft rotates the cam to move the arm.

15. The device of claim 1, wherein the conduit positioner comprises

an arm pivotable about an axis, the deposition conduit is coupled to the arm, pivoting of the arm about the axis in a first direction moves the exit end of the deposition conduit toward the substrate supported by the substrate positioner and pivoting of the arm about the axis in a second direction moves the exit end of the deposition conduit away from the substrate supported by the substrate positioner.

16. The device of claim 15, wherein the substrate positioner comprises an X-Y table that is operable to move the substrate in an x-direction and a y-direction.

17. The device of claim 15, wherein the substrate positioner comprises a reel-to-reel tape drive that is operable to move the substrate from a first reel to a second reel.

18. The device of claim 15, further comprising a fluid treatment device coupled to the deposition conduit.

19. The device of claim 18, wherein the fluid treatment device operates to output the effluent into the deposition conduit in a form suitable for matrix-assisted laser desorption ionization.

20. The device of claim 15, further comprising a motor having an output shaft and a cam coupled to the output shaft, the arm engages the cam, and rotation of the output shaft rotates the cam to pivot the arm.

21. The device of claim 15, further comprising one of a linear stepper motor or a solenoid that is coupled to the arm and that is operable to pivot the arm.

8. A device for placement of effluent on substrate to be subsequently used in matrix-assisted laser desorption ionization mass spectroscopy (MALDI MS) analysis, the device comprising

a substrate positioner configured to support and position the substrate,

a deposition conduit having a first opening through which effluent enters the conduit and having an exit end with a second opening through which effluent exits the conduit, and

a conduit positioner configured to move the exit end of the deposition conduit toward the substrate to a position in which a quantity of effluent is transferred away from the exit end and is deposited on the substrate as a result of the quantity of effluent contacting and adhering to the substrate, the exit end of the deposition conduit being exposed to the ambient atmosphere.

9. The device of claim 8, wherein the substrate positioner comprises an X-Y table that is operable to move the substrate in an x-direction and a y-direction.

10. The device of claim 8, wherein the substrate positioner comprises a reel-to-reel tape drive that is operable to move the substrate from a first reel to a second reel.

11. The device of claim 8, further comprising a fluid treatment device coupled to the deposition conduit.

12. The device of claim 11, wherein the fluid treatment device operates to mix a matrix material with an analyte to form the effluent to be deposited on the substrate.

13. The device of claim 8, wherein the conduit positioner comprises an arm, the deposition conduit is coupled to the aim, and the arm is pivotable to move the exit end of the deposition conduit to the position in which effluent is deposited on the substrate.

14. The device of claim 13, wherein the conduit positioner further comprises a motor having an output shaft and a cam coupled to the output shaft, the arm engages the cam, and rotation of the output shaft rotates the cam to pivot the arm.

22. An apparatus comprising

a moveable, controllable substrate positioner configured to support and position a substrate,

a conduit having a first opening through which effluent enters the conduit and having an exit end with a second opening through which effluent emerges from the conduit,

a moveable, controllable conduit positioner configured to support and position the conduit from which a sample of effluent emerges for deposition on the substrate, the sample being transferred away from the exit end and deposited on the substrate as a result of the exit end of the conduit being moved to a position in which the sample contacts and adheres to the substrate,

at least one controller coupled to the substrate positioner and the conduit positioner to control the positioning of the substrate positioner and the conduit positioner, and

a computer device having a database which is configured to store information about a source from which the sample was taken and information about test results from at least one test of the sample conducted after deposition of the sample on the substrate.