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Methods and apparatus for analyzing polynucleotide sequences

  • US 6,911,345 B2
  • Filed: 07/18/2001
  • Issued: 06/28/2005
  • Est. Priority Date: 06/28/1999
  • Status: Expired due to Fees
First Claim
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1. An apparatus for analyzing the sequence of a polynucleotide, comprising:

  • (a) a flow cell comprising at least one synthesis channel coated with a polyelectrolyte multilayer;

    (b) an inlet port and an outlet port in a fluid communication with said flow cell for flowing fluids into and through said flow cell and said at least one synthesis channel; and

    (c) at least one polynucleotide attached to said polyelectrolyte multilayer, wherein said polyelectrolyte multilayer comprises alternating at least one polycation and at least one polyanion layers and wherein said at least one polynucleotide is attached to said polyanion layer.

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