2′ Modified oligonucleotides
First Claim
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1. A mixed sequence oligonucleotide having the formula:
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or a salt thereof, wherein;
each B independently is a protected or unprotected purine or pyrimidine base in the β
-anomeric configuration;
W3 and W4 are each independently H, PO3−
2, a protecting group, or an intermediate moiety in the formation of an internucleotide link which when reacted with the appropriate —
OH results in Z;
n is an integer of 1-200;
each Z is independently P(O)O, P(O)S or P(O)N(R)2;
each R is independently H or alkyl (C1-6);
each A is H,OH, O—
Pr wherein Pr is a protecting group, or X-Y wherein X-Y is O-(unsubstituted alkyl (C2-20)), S-(unsubstituted alkyl (C1-20)), or NH-(unsubstituted alkyl (C1-20)); and
at least one A is X-Y.
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Abstract
Oligomers which have substituents on the 2′ position are resistant to oligonucleases and furthermore can be derivatized to deliver reagents or drugs, to carry label, or to provide other properties.
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Citations
14 Claims
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1. A mixed sequence oligonucleotide having the formula:
-
or a salt thereof, wherein; each B independently is a protected or unprotected purine or pyrimidine base in the β
-anomeric configuration;
W3 and W4 are each independently H, PO3−
2, a protecting group, or an intermediate moiety in the formation of an internucleotide link which when reacted with the appropriate —
OH results in Z;
n is an integer of 1-200;
each Z is independently P(O)O, P(O)S or P(O)N(R)2;
each R is independently H or alkyl (C1-6);
each A is H,OH, O—
Pr wherein Pr is a protecting group, or X-Y wherein X-Y is O-(unsubstituted alkyl (C2-20)), S-(unsubstituted alkyl (C1-20)), or NH-(unsubstituted alkyl (C1-20)); and
at least one A is X-Y.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification