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Isolation of nucleic acids

  • US 6,914,137 B2
  • Filed: 12/14/2000
  • Issued: 07/05/2005
  • Est. Priority Date: 12/06/1997
  • Status: Expired due to Term
First Claim
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1. A product comprising a solid phase for use in a method in which the solid phase reversibly binds nucleic acid present in a sample, the product comprising a plurality of positively ionizable groups, wherein the ionizable groups are immobilized on the solid phase and are effective at a first pH at which the ionizable groups are positively charged to bind nucleic acid present in a sample and are effective to release the nucleic acid at a second, higher, pH at which the charge on the ionizable groups is negative, neutral or less positive, the ionizable groups being provided by a chemical species selected from the group consisting of biological buffers, polyhydroxylated amines, histidine and polyhistidine, wherein said plurality of positively ionizable groups have a pKa between about 4.5 and about 8.5.

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