Method of matching retention times among multiple chromatographic system

• US 6,915,227 B2
• Filed: 11/26/2003
• Issued: 07/05/2005
• Est. Priority Date: 11/30/2001
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Dryden, Paul C., Axelson, Elmer A., Klee, Matthew S., Quimby, Bruce D.
• Primary Examiner(s): Barlow, John
• Assistant Examiner(s): Bhat, Aditya S
• Application Number:
  US10/722,994
• Publication Number:
  US 20040122611A1
• Time in Patent Office: 587 Days
• Field of Search: 702/22, 702/89, 73/233.5, 703/2, 600/558, 435/6
• US Class Current: 702/89
• CPC Class Codes: G01N 30/8665 for calibrating the measuring apparatus
• CPC Class Codes: G01N 30/8672 not depending on an individual instrument, e.g. retention time indexes or calibration transfer

Abstract

A data manipulation method to make local chromatography data more usable and comparable to a reference. The method provides time axis correction to better match local data to a reference of the same time scale, time axis transformation to correspond more directly to a reference based on a different time scale, and response axis correction to better match a reference of the same or different response scale, while maintaining the original peak areas. The method may be used along or concurrently with other data manipulation technique to facilitate operations such as searching, matching, visual comparison, mathematical manipulation, and pattern recognition of chromatographic data.

Claims

1. A process for correcting a time axis of local chromatographic data to match reference chromatographic data while maintaining peak areas, comprising:

obtaining reference calibration data from a reference chromatographic system;

obtaining local calibration data from a local chromatographic system;

generating local system correction functions based on an appropriate mathematical relationship using the reference calibration and the local calibration data; and

applying the local system correction functions to local chromatographic data to generate corrected local chromatographic data,

wherein the corrected local chromatographic data match with the reference calibration data on time axis and wherein peak areas in the local chromatographic data are maintained in the corrected local chromatographic data, wherein the local system correction functions include a smoothed x-axis correction function and a y-axis correction function generated using one or more mathematical curve fitting techniques, said y-axis correction function is a first derivative of the x-axis correction function.

2. The process of claim 1, wherein the one or more mathematical curve fitting techniques are selected from the group consisting of linear regression, polynomial regression, logarithmic regression, cubic splining, and exponential regression.

3. The method of claim 1, wherein the local system correction functions are applied to the local chromatographic data through steps of:

(1) determining a retention time correction (Δ

rt) from the smoothed x-axis correction function for a given reference retention time (rt);

(2) copying a y value that is at retention time (rt+Δ

rt) in the local chromatographic data to rt;

(3) multiplying copied y value by (1+dΔ

rt/drt), wherein dΔ

rt/drt is determined from the first derivative of the smoothed x-axis correction function;

repeating steps (1) to (3) with each retention time data point in the local chromatographic data to create corrected local chromatographic data.

4. The process of claim 1, further comprising:

replacing a y-axis unit in at least one of the reference calibration data and the local calibration data with a new y-axis unit.

5. The process of claim 4, wherein the new y-axis unit is concentration, percent, weight, mass, moles, or mole fraction.

6. The process of claim 1, further comprising:

applying the local system correction functions to adjust the local calibration data and to generate time-axis correct local calibration data that maintains the peak areas of the local calibration data;

determining a y-axis local response correction function using the time-axis correct local calibration data and the reference calibration data;

applying the y-axis local system response correction function to the local chromatographic data.

7. The process of claim 1, wherein generation of at least one of the local system correction functions and the corrected local chromatographic data is performed at a remote location through a network.

8. The process of claim 7, wherein the network is a local network or the Internet.

9. A process for correcting local chromatographic data with a reference retention time database, comprising:

providing a reference retention time database;

running a calibration mix on the local system to generate location calibration data;

constructing local system correction functions to minimize differences between the local calibration data and corresponding retention time values in the reference retention time database for the calibration mix;

applying the local system correction functions to adjust local chromatographic data; and

using adjusted local chromatographic data to search the reference retention time database to obtain more accurate search results.

10. The process of claim 9 further comprising:

locking a local system to the reference retention dine database using an instrumental method.

11. The process of claim 10, wherein the instrumental method is RTL I method.

12. A process for correcting local chromatographic data by generating a local retention time database from a reference retention time database, comprising:

providing a reference retention time database;

locking a local system to the reference retention time database using an instrumental method;

running a calibration mix on the local system to generate local calibration data;

constructing local system correction functions to minimize differences between the local calibration data and corresponding retention time values in the reference retention time database for the calibration mix;

applying the local system correction functions to adjust the reference retention time database values to generate a local retention time database; and

searching the local retention time database using local chromatographic data.

13. A chromatographic apparatus for analyzing samples, comprising:

means for producing local chromatographic data; and

means for generating corrected local chromatographic data that match with reference chromatographic data on a time axis while maintaining peak areas of the local chromatographic data, further comprising;

means for creating local system correction functions based on an appropriate mathematical relationship using reference calibration data local calibration data;

means for applying the local system correction functions to local chromatographic data;

means for replacing a y-axis unit in at least one of the reference calibration data and local calibration data with a new y-axis unit.

14. The chromatographic apparatus of claim 13, wherein the means for generating corrected local chromatographic data further comprising:

means for determining a y-axis local system response correction function; and

means for applying the y-axis local system response correction function to the local chromatographic data.