Cultured skin and method of manufacturing the same

• US 6,916,655 B2
• Filed: 11/21/2002
• Issued: 07/12/2005
• Est. Priority Date: 11/22/2001
• Status: Expired due to Fees

• Current Assignee: Nipro Corporation
• Original Assignee: Nipro Corporation
• Inventors: Yasumoto, Shigeru, Takeuchi, Masakatsu
• Primary Examiner(s): Afremova, Vera
• Application Number:
  US10/300,906
• Publication Number:
  US 20030096409A1
• Time in Patent Office: 964 Days
• Field of Search: 435/371, 435/366, 435/383, 435/404, 435/1.1, 435/395, 623/15.12, 623/901
• US Class Current: 435/371
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3813 Epithelial cells, e.g. keratinocytes, urothelial cells
• CPC Class Codes: A61L 27/3826 Muscle cells, e.g. smooth muscle cells
• CPC Class Codes: A61L 27/383 Nerve cells, e.g. dendritic cells, Schwann cells
• CPC Class Codes: A61L 27/3891 as distinct cell layers
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: C12N 2502/094 keratinocytes
• CPC Class Codes: C12N 2502/1323 Adult fibroblasts
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0698 Skin equivalents

Abstract

A cultured skin and a grafting cultured skin sheet are provided, each of which is a cultured reconstructive skin with a high take rate using cells collectable from cells originated from tissue included in an umbilical cord such as tissue included in an umbilical cord originated from a human fetus. The grafting cultured skin stratified sheet is prepared by placing an epithelium sheet on the top surface of a cultured dermis. The cultured dermis includes as components a cultured skin containing cells originated from a tissue included in an umbilical cord, such as umbilical cells, more concretely, umbilical fibroblast cells, being separated and cultured, preferably in a collagen nonwoven fabric. On the other hand, the epithelium sheet is prepared by culturing and stratifying the umbilical cord epithelium cells.

Claims

1. A method of culturing umbilical cord epithelium cells, characterized by culturing in a medium umbilical cord epithelium cells separated from cells originated from tissue included in an umbilical cord, wherein the medium is a mixture of MCDB153 medium and DMEM medium in a volume ratio of 4:

1 supplemented with epidermal growth factor, insulin, transferrin, hydrocortisone, ethanol amine, phosphoethanolamine and hypophysis extract.