Human glandular kallikrein enhancer, vectors comprising the enhancer and methods of use thereof
First Claim
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1. An isolated polynucleotide comprising nucleotides about 8021 to about 8371 of SEQ ID NO:
- 1, or a fragment thereof of comprising bases 8192-8206 of SEQ ID NO;
1, wherein said polynucleotide or said fragment thereof has prostate-specific enhancer activity.
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Abstract
Enhancers which preferentially increase the transcription of cis-linked coding sequences in prostate cells are provided. Methods of using DNA constructs comprising the enhancers to control transcription of heterologous polynucleotides are also provided. Delivery vehicles comprising the enhancers and methods of using the vehicles are also provided. Adenovirus vectors in which one or more genes are under transcriptional control of the enhancers of the invention are also provided. Further provided are methods of using the adenovirus vectors of the invention to confer selective cytotoxicity in mammalian cells.
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14 Claims
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1. An isolated polynucleotide comprising nucleotides about 8021 to about 8371 of SEQ ID NO:
- 1, or a fragment thereof of comprising bases 8192-8206 of SEQ ID NO;
1, wherein said polynucleotide or said fragment thereof has prostate-specific enhancer activity.
- 1, or a fragment thereof of comprising bases 8192-8206 of SEQ ID NO;
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2. An isolated polynucleotide comprising nucleotides about 8021 to about 8371 of SEQ ID NO:
- 1, wherein said polynucleotide has prostate-specific enhancer activity.
- View Dependent Claims (3, 4, 5, 6)
- 7. An adenovirus vector for selective cytolysis of a prostate target cell comprising an adenovirus gene essential for replication under transcriptional control of a human glandular kallikrein transcription regulatory element (hKLK2-TRE), wherein said hKLK2-TRE comprises a promoter and nucleotides about 8021 to about 8371 of SEQ ID NO:
Specification