Method for detection of pathogenic microorganisms
First Claim
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1. A method for forming an image of an organic object comprising:
- a) illuminating said object with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological;
b) forming an image of said organic object using Raman shifted light from said object;
c) analyzing said Raman shifted light image for patterns characteristic of said organic object.
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Abstract
Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.
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Citations
26 Claims
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1. A method for forming an image of an organic object comprising:
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a) illuminating said object with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological;
b) forming an image of said organic object using Raman shifted light from said object;
c) analyzing said Raman shifted light image for patterns characteristic of said organic object. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for forming an image of one of a plurality of organic objects comprising:
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a) providing said plurality of organic objects in a bio-dilute sample;
b) illuminating said sample with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological;
c) forming an image of one of said plurality of organic objects using Raman shifted light from said imaged organic object;
d) analyzing said Raman shifted light image for patterns characteristic of said organic object. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for forming an image of one of a plurality of pathogenic microorganisms comprising:
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a) providing a bio-dilute sample of said plurality of pathogenic microorganisms;
b) illuminating said bio-dilute sample with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological;
c) forming an image of one of said plurality of pathogenic microorganisms using Raman shifted light from said imaged pathogenic microorganism;
d) analyzing said Raman shifted light image for patterns characteristic of said one of said plurality of pathogenic microorganisms.
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26. A method for forming an image of an organic object comprising:
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a) illuminating a biodilute sample including said organic object with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological;
b) forming an image of said illuminated organic object using Raman shifted light from said object;
c) analyzing said Raman shifted light image for patterns characteristic of said organic object.
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Specification