Method and apparatus for conducting an array of chemical reactions on a support surface

• US 6,921,636 B1
• Filed: 11/16/2000
• Issued: 07/26/2005
• Est. Priority Date: 09/04/1991
• Status: Expired due to Fees

• Current Assignee: Metrigenix Incorporated
• Original Assignee: Metrigenix Incorporated
• Inventors: Brennan, Thomas M.
• Primary Examiner(s): FREDMAN, JEFFREY NORMAN
• Application Number:
  US09/715,426
• Time in Patent Office: 1,713 Days
• Field of Search: 435/6, 435/91.2, 435/283.1, 435/285.1, 422/57, 422/58, 536/334, 536/335, 536/336
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00639 the compounds being trapped in or bound to a porous medium
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C03C 17/30 : with silicon-containing compounds
• CPC Class Codes: C07B 2200/11 : Compounds covalently bound to a solid support
• CPC Class Codes: C07B 61/00 : Other general methods
• CPC Class Codes: C07H 21/00 : Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07K 1/045 : using devices to improve synthesis, e.g. reactors, special vessels
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6874 : involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

The invention provides apparatus and methods for determining the nucleotide sequence of target nucleic acids using hybridization to arrays of oligonucleotides. The invention further provides apparatus and methods for identifying the amino acid sequence of peptides that bind to biologically active macromolecules, by specifically binding biologically active macromolecules to arrays of peptides or peptide mimetics.

Claims

1. A solid support comprising a support surface with an array of functionalized sites wherein a solution in the amount of about 50 picoliter to 2 microliter at a functionalized site is separated from solutions at other functionalized sites by surface tension, and wherein the support surface of the functionalized site has a higher surface tension relative to the site surface surrounding the functionalized site.

2. The solid support of claim 1 wherein said support surface has 10-10⁴ functionalized sites per cm².

3. The solid support of claim 1 wherein said functionalized site is about 50-2000 microns in diameter.

4. The solid support of claim 1 wherein said support surface is glass.

5. The solid support of claim 1 wherein said support surface is selected from the group consisting of nylon, polyethylene, polypropylene, polystyrene and polytetraflourelene.

6. The solid support of claim 1 wherein said functionalized site provides attachment to a nucleic acid.

7. The solid support of claim 1 wherein said functionalized site provides attachment to a peptide.

8. The solid support of claim 1, 6 or 7 wherein said functionalized site provides a covalent attachment.

9. The solid support of claim 1, 6 or 7 wherein said functionalized site provides a non-covalent attachment.