Patched genes and uses related thereto

• US 6,921,646 B2
• Filed: 01/03/2001
• Issued: 07/26/2005
• Est. Priority Date: 10/07/1994
• Status: Expired due to Fees

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Inventors: Johnson, Ronald L., Goodrich, Lisa V., Scott, Matthew P.
• Primary Examiner(s): Kemmerer, Elizabeth
• Assistant Examiner(s): BRANNOCK, MICHAEL T
• Application Number:
  US09/754,032
• Publication Number:
  US 20030148388A1
• Time in Patent Office: 1,665 Days
• Field of Search: 435/7.21, 435/69.1, 435/252.3, 435/320.1, 435/7.1, 436/501, 514/2, 530/350, 530/388.22, 536/23.5
• US Class Current: 435/7.21
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/43563 from insects
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

Invertebrate and vertebrate patched genes are provided, including the mouse and human patched genes, as well as methods for isolation of related genes, where the genes may be of different species or in the same family. Having the ability to regulate the expression of the patched gene, allows for the elucidation of embryonic development, cellular regulation associated with signal transduction by the patched gene, the identification of agonist and antagonist to signal transduction, identification of ligands for binding to patched, isolation of the ligands, and assaying for levels of transcription and expression of the patched gene.

Claims

1. A method of screening for compounds that bind to a patched protein, said method comprising:

(a) combining a candidate compound with a cell comprising an expression cassette further comprising (i) a transcriptional initiation region, (ii) a transcriptional termination region, and (iii) a nucleic acid sequence encoding the patched protein under the transcriptional regulation of said transcriptional initiation region and said transcriptional termination region, wherein said nucleic acid sequence (I) encodes the patched protein, which patched protein is present in the membrane of said cell and (II) hybridizes under stringent conditions, including a wash step of 0.2×

SSC at 65°

C., to the nucleic acid sequence represented in SEQ ID NO;

18, and wherein the patched protein can bind to a hedgehog polypeptide; and

(b) assaying for the binding of said candidate compound to the patched protein.

2. The method of claim 1, wherein said cell is a mammalian cell.

3. The method of claim 1, wherein the patched protein is encoded by a nucleic acid sequence represented in SEQ ID NO:

18.

4. The method of claim 1, wherein the patched protein comprises an amino acid sequence represented in SEQ ID NO:

19.