Cyclodextrin glucanotransferase and its method of manufacture
First Claim
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1. Cyclodextrin glucanotransferase having the enzymatic chemical properties listed below:
- a) function and substrate specificity;
enzymatically acting on starch, dextrin, amylopectin, and amylose to produce primarily γ
-cyclodextrin, with the quantities of β
- and α
-cyclodextrin produced being smaller than the quantity of γ
-cyclodextrin produced;
b) optimum pH;
10.5-11.0;
c) optimum temperature;
about 60°
C.;
d) stable pH;
6-11;
e) temperature stability;
with a 15 minute-treatment at 50°
C., residual activity of not less than 90 percent is exhibited.
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Abstract
CGTase enzymatically acts on starch, dextrin, amylopectin, and amylose to produce primarily γ-CD, with quantities of β- and α-CD produced being smaller than the quantity of γ-CD produced, has an optimum pH of 10.5-11.0, an optimum temperature of about 60° C., a stable pH of 6-11, and temperature stability, exhibits residual activity of not less than 90 percent with a 15 minute-treatment at 50° C., is produced by culturing a Bacillus clarkii (for example, FERM B-7156), reacts with starch or the like to produce principally γ-cyclodextrin.
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Citations
7 Claims
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1. Cyclodextrin glucanotransferase having the enzymatic chemical properties listed below:
-
a) function and substrate specificity;
enzymatically acting on starch, dextrin, amylopectin, and amylose to produce primarily γ
-cyclodextrin, with the quantities of β
- and α
-cyclodextrin produced being smaller than the quantity of γ
-cyclodextrin produced;
b) optimum pH;
10.5-11.0;
c) optimum temperature;
about 60°
C.;
d) stable pH;
6-11;
e) temperature stability;
with a 15 minute-treatment at 50°
C., residual activity of not less than 90 percent is exhibited.
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- 2. A method of manufacturing cyclodextrin glucanotransferase wherein a microorganism belonging to the species Bacillus clarkii that produces cyclodextrin glucanotransferase is cultured to produce cyclodextrin glucanotransferase, and the cyclodextrin glucanotransferase that has been produced is collected.
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4. A method of manufacturing cyclodextrin wherein cyclodextrin glucanotransferase produced by the species Bacillus clarkii is reacted with a solution comprising at least one member selected from the group consisting of starch, dextrin, amylopectin, and amylose to produce principally γ
- -cyclodextrin.
- View Dependent Claims (5, 6)
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7. Bacillus clarkii strain 7364 (FERM BP-7156) being the species Bacillus clarkii having the ability to produce cyclodextrin glucanotransferase.
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