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Expression monitoring by hybridization to high density oligonucleotide arrays

  • US 6,927,032 B2
  • Filed: 01/28/2003
  • Issued: 08/09/2005
  • Est. Priority Date: 09/15/1995
  • Status: Expired due to Fees
First Claim
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1. A method for monitoring hybridization of an eukaryotic nucleic acid sample to a nucleic acid array, said method comprising the steps of:

  • (a) providing a nucleic acid array comprising a plurality of nucleic acid test probes that specifically bind to eukaryotic nucleic acid targets and at least one control probe that specifically binds to a bacterial gene;

    (b) providing a spiked sample comprising an eukaryotic nucleic acid sample with a nucleic acid control, said control comprising a nucleic acid that specifically binds to the control probe;

    (c) contacting said spiked sample with said array; and

    (d) determining whether hybridization occurred between the control probe and the nucleic acid control.

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