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Methods and apparatus for template capture and normalization for submicroliter reaction

  • US 6,927,045 B2
  • Filed: 09/30/2002
  • Issued: 08/09/2005
  • Est. Priority Date: 08/02/1999
  • Status: Expired due to Fees
First Claim
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1. A method of performing an enzymatic reaction in a capillary tube using a normalized quantity of DNA, comprising:

  • introducing an enzymatic reaction mixture into a capillary tube, said tube having a normalized quantity of DNA, wherein said reaction mixture comprises an oligonucleotide primer, a DNA polymerase, and deoxynucleotide triphosphates (dNTPs), said DNA having been saturably bound from an excess thereof directly on an inner surface of said capillary tube by contacting said inner surface with a solution comprising DNA and a chaotropic agent for a time sufficient for the DNA to have become saturably bound to said inner surface; and

    said excess of DNA having been removed therefrom; and

    performing said enzymatic reaction in said capillary tube using said normalized quantity of DNA.

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