Device and method for separation

• US 6,929,750 B2
• Filed: 03/11/2002
• Issued: 08/16/2005
• Est. Priority Date: 03/09/2001
• Status: Expired due to Term

• Current Assignee: ErySave AB
• Original Assignee: ErySave AB
• Inventors: Nilsson, Andreas, Petersson, Filip Tobias, Persson, Hans W., Jönsson, Henrik, Laurell, Thomas, Allers, Mats
• Primary Examiner(s): LAWRENCE JR, FRANK M
• Application Number:
  US10/467,843
• Publication Number:
  US 20040069717A1
• Time in Patent Office: 1,254 Days
• Field of Search: 210/748, 210/708, 210/767, 210/95, 209/155, 422/20, 422/128, 204/157.15, 204/158.2, 204/193
• US Class Current: 210/708
• CPC Class Codes: A61M 1/3472 with treatment of the filtrate
• CPC Class Codes: A61M 1/3479 by dialysing the filtrate
• CPC Class Codes: A61M 1/3496 Plasmapheresis; Leucopheresis; Lymphopheresis A61M1/3472 takes precedence
• CPC Class Codes: A61M 1/3678 Separation of cells using wave pressure; Manipulation of individual corpuscles

Abstract

The present invention provides a device and a method for separating particles from fluids using ultrasound, laminar flow, and stationary wave effects comprising a micro-technology channel system with an integrated branching point or branching fork, and a single ultrasound source. One of the characteristics of the invention is that the single ultrasound source, which generates the standing waves, excites the complete structure including the channel system. No special reflectors or the like are needed. Extremely thin dividers can separate the flow, thereby enhancing the effectiveness of the device. The device could be manufactured in silicon and the ultrasound energy could preferably be delivered by a piezoelectric element.

Claims

1. A device for separating suspended particles from a fluid, comprising a channel unit arranged in a plate having first and second opposing great surfaces, said channel unit including a base stem channel having substantially parallel or near parallel base stem walls perpendicular to said surfaces, said base stem channel having an inlet and, opposite said inlet, a branching point connected to two or more different outlets;

and oscillation means for delivering mechanical energy to a fluid in said channel unit, such that said particles are concentrated into laminar layers in the base stem channel, substantially parallel to said base stem walls, wherein said branching point is devised to separate particles, arranged in said laminar layers in a fluid flowing in said base stem channel, to said different outlets, wherein said channel unit is formed as a part of a material layer close to said first great surface, and said oscillation means are arranged in contact with said second great surface for delivering mechanical energy to said plate such that a standing wave field is created between said base stem walls.

2. A device according to claim 1, wherein said oscillation means are arranged to deliver mechanical energy in a direction perpendicular to the first and second surfaces of said plate.

3. A device according to claim 1 further comprising a control unit capable of controlling said oscillation means to deliver mechanical energy of controlled frequency and power within the ultrasound frequency band and with the frequency being so adapted to the dimensions of the channel unit that in a width of the channel, between base stem walls, an acoustic standing wave field is created.

4. A device according to claim 1, wherein a number of channel units are arranged in the same plate receiving mechanical energy from a single oscillation means allowing for integration of a large number of channel units for separation purposes on a single plate.

5. A device according to claim 1, wherein the channel unit is provided with an inlet and three outlets.

6. A device according to claim 1, wherein the plate comprises a piece of homogenous material in which said channel unit is defined.

7. A device according to claim 6, wherein the first surface of said plate is covered by a layer of glass.

8. A device according to claim 7, wherein said plate and said layer of glass are bonded together.

9. A device according to claim 1, wherein said plate is made of silicon.

10. A device according to claim 1, wherein said plate is made of plastic.

11. A device according to claim 1, wherein the branching point is shaped like a cross, and the inlet is located at the lower end of the cross base stem and the three outlets are located at the top of the cross.

12. A device according to claim 1, wherein the branching point divides the base stem into three arms with angles α

1 and α

2 between them, and that the value of α

1 and α

2 are between 0 and 90 degrees.

13. A device according to claim 1, wherein the branching point comprises the division of the base stem directly into three parallel channels divided by thin dividing walls.

14. A device according to claim 13 wherein the thin walls have a thickness of between 1 and 40 micrometer, preferably 20 micrometer.

15. A device according to claim 1, wherein the width of the channel is in the range between 60 and 1400 micrometer.

16. A device according to claim 1, wherein the width of the channel is 700 micrometer.

17. A device according to claim 1, wherein said oscillation means comprises a piezoelectric element.

18. A device according to claim 17, wherein said mechanical energy is of controlled frequency and power inside the ultrasound frequency band.

19. A device according to claim 18, wherein the electrical energy is controllable with regard to waveform, frequency and power.

20. A device according to claim 19, wherein the waveform is controllable to be one of but not limited to sinus wave, triangular wave or square wave.

21. A device according to claim 1, wherein the dimensions of the channel unit, i.e. the width and a height of the channel, the frequency of the oscillation means and a flow rate is adapted to accommodate blood as said fluid and the red blood cells as the particles to be separated from the fluid.

22. A device according to claim 1, wherein the dimensions of the channel unit, i.e. the width and a height of the channel, the frequency of the oscillation means and a flow rate is adapted to handle a fluid containing particles of biological material containing fat.

23. A device according to claim 1, wherein the channel unit is provided with three inlets and three outlets.

24. A device according to claim 22, wherein the dimensions of the channel unit, i.e. the width and a height of the channel, the frequency of the oscillation means are adapted to handle a fluid containing platelets.

25. A separator unit for use in a device according to claim 1, wherein said separator unit comprises a plate having first and second opposing great surfaces, and in that a channel unit is formed as a part of a material layer close to said first great surface, said channel unit including a base stem channel having substantially parallel or near parallel base stem walls perpendicular to said surfaces, said base stem channel having an inlet and, opposite said inlet, a branching point connected to two or more different outlets, and wherein said second surface is connectable to oscillation means for delivering mechanical energy to a fluid in said channel unit wherein a number of channels units are arranged in the same plate, for receiving mechanical energy from a single oscillation means.

26. A separator unit according to claim 25, wherein the plate comprises a piece of homogenous material in which said channel unit is defined.

27. A separator unit according to claim 26, wherein said homogenous material is plastic.

28. A separator unit according to claim 26, wherein said homogeneous material is silicon.

29. A separator unit according to claim 26, wherein the first surface of said plate is covered by a sealing layer which makes it possible to visually inspect a separation process in said channel.

30. A separator unit according to claim 29, wherein the sealing layer is made of glass.

31. A separator unit according to claim 30, wherein said plate and said layer of glass are bonded together.

32. A method for separating particles from fluids using ultrasound, laminar flow, and stationary wave effects comprising the steps of:

feeding a fluid to a separator unit comprising a plate having first and second opposing great surfaces and a channel unit formed as a part of a material layer close to said first surface, forcing the fluid to a substantially laminar flow in a flow direction;

applying an ultrasound oscillating wave field to said second surface, thereby subjecting said flow to an ultrasound stationary wave field during its flow past a distance in said channel unit, forcing said particles to a non-uniform distribution in a separation direction parallel to said surfaces and perpendicular to the flow direction; and

separating said second laminar flow into a first and a second separated flow in such a way that the concentration of particles is higher in the first separated flow than in the second separated flow.

33. The method according to claim 32, wherein said ultrasound oscillating wave field is given a frequency adapted to a width of the channel unit, such that vibrations in the plate give rise to said wave field parallel with the plate.

34. The method according to claim 32, wherein said ultrasound oscillating wave field is applied perpendicular to said surfaces of the plate.

35. The method according to claim 32, further comprising the step of separating out particles of biological material containing fat from a fluid.

36. The method according to claim 32, further comprising the step of separating particles from blood.

37. The method as recited in claim 32, further comprising the step of separating out bacteria from a fluid.

38. The method as recited in claim 32, further comprising the step of separating out stem cells from a fluid.

39. The method as recited in claim 32, further comprising the step of separating out platelets from a fluid.

40. The method as recited in claim 32, further comprising the step of adding a solution to the original fluid, said solution having a different density than the original fluid, with the purpose of altering the density of the fluid from which particles are to be separated.

41. The method according to claim 32, wherein the method is repeated in a number of stages.

42. The method according to claim 41, wherein new fluid is introduced before the steps are repeated.

43. The method according to claim 32, further comprising the step of controlling the power fed to the ultrasound stationary wave field by means of controlling the electrical energy with regard to waveform, frequency and power to a piezoelectric element transmitting its mechanical energy to the fluid and its surroundings.