Method of controlling the temperature of a specimen in or on a solid support member

• US 6,930,292 B1
• Filed: 07/21/2000
• Issued: 08/16/2005
• Est. Priority Date: 07/21/1999
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Dako A/S (Agilent Technologies, Inc.)
• Inventors: Winther, Lars, Adelhorst, Kim
• Primary Examiner(s): Van, Quang T.
• Application Number:
  US10/031,357
• Time in Patent Office: 1,852 Days
• Field of Search: 219/635, 219/647, 219/201, 219/50, 435/440, 435/69.1, 435/173.1, 436/35, 324/300, 356/311, 216/67, 156/345.38, 156/345.48, 204/298.31, 204/298.34, 204/157, 204/44
• US Class Current: 219/635
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: G01N 1/44 Sample treatment involving radiation, e.g. heat
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A method of controlling the temperature of a biological specimen by using induction heating. The specimen is either fixed to a carrier or is in liquid form in contact with a carrier with fixed capture probes. The carrier is removably placed in proximity to a solid support member with a conducting material, and the solid support is subjected to an oscillating magnetic field. The carrier and support member are preferably a microscope slide and a cartridge, respectively.

Claims

1. A method for controlling the temperature of a biological specimen in indirect contact with a solid support member by using induction heating and a carrier for a biological specimen, said carrier being removably placed in proximity to said solid support member, said solid support member includes a cartridge for said carrier or a cover plate for said carrier and comprising an electrically conducting material, said electrically conducting material being in contact with a layer of heat conducting material, said heat conducting material is in contact with the specimen, and said method comprising a step of subjecting said solid support to an oscillating magnetic field.

2. A method according to claim 1, wherein said solid support member includes a cartridge having a chamber encompassed by a cartridge wall, said carrier being placed in said chamber and said chamber being subjected to a magnetic field, said chamber includes at least one access opening for introducing the carrier, and for passing a processing fluid into and out of the chamber.

3. A method according to claim 2 wherein said conducting material includes the form of a solid piece of electrically conducting material placed on the inner side of said cartridge wall, or the form of one or more solid pieces or particles of electrically conducting material incorporated in the wall of said cartridge.

4. A method according to claim 2, wherein said carrier includes a microscope slide, said cartridge comprising a chamber, and at least one access opening for introducing and withdrawing said slide, and having at least one opening for passing a processing fluid into and out of the chamber, said microscope slide is placed in said chamber.

5. A method according to claim 1, wherein said carrier comprises a microscope slide bearing a specimen or capture probes for capturing a specimen said solid support member includes a cover plate for said microscope slide, said cover plate comprising an electrically conducting material, said specimen or said capture probes being fixed onto said microscope slide and placed between said cover plate and said slide when subjecting said solid support to an oscillating magnetic field.

6. A method according to claim 1, wherein the electrically conducting material includes a metal.

7. A method according to claim 6, wherein said metal is selected from a group consisting of iron, carbon steel, stainless steel, brass, copper, aluminum, silver, gold, platinum, nickel, zinc, pewter and alloys thereof.

8. A method according to claim 1, wherein the electrically conducting material is in the form of one or more plates, having a length, a width, and a thickness, said length and said width being at least ten times the thickness.

9. A method according to claim 1, wherein the electrically conducting material is in the form of powder incorporated in a polymer material, the amount of powder being sufficiently high to raise the temperature of the specimen when the solid support is subjected to the oscillating magnetic field.

10. A method according to claim 9, wherein said specimen is in the form of a solid specimen, preferably a tissue section or a section of cell blocks.

11. A method according to claim 1, wherein said solid support includes an amount of electrically conducting material sufficiently high to raise the temperature of the specimen when the solid support is subjected to the oscillating magnetic field.

12. A method according to claim 1, wherein said magnetic field is generated by use of an electromagnetic inductor having an induction coil and a power supply, and directing alternating current through said coil.

13. A method according to claim 12, wherein said power supply includes an alternating current power supply.

14. A method according to claim 12, wherein said alternating current power includes a frequency in the range of between 1 Hz and 500 kHz.

15. A method according to claim 12, wherein alternating current is delivered through said coil in an amount of power up to about 100 W.

16. A method according to claim 1, comprising a step of heating the specimen to a temperature in the range of between 25 and 110°

C.

17. A method according to claim 1, wherein the specimen is heated and maintained at a constant temperature for a period in the range of between one minute and up to one week.

18. A method according to claim 1, wherein the specimen is dried or fixed or both at an elevated temperature.

19. A method according to claim 1, wherein the specimen is subjected to a reaction step at an elevated temperature, said reaction step includes one or more of the steps capturing the specimen, baking the specimen, exposing the specimen to antigen retrieval, denaturing the specimen, hybridizating the specimen, dewaxing the specimen and washing the specimen.

20. Use of the method according to claim 1 for treatment of a biological specimen.

21. Use of the method according to claim 1 for immunohistochemical procedures or in situ hybridization.

22. A method for carrying out an automatic or semi-automatic assay of one or more specimens each fixed on a microscope slide, comprising the steps of:

i) placing the microscope slide in a cartridge comprising a chamber encompassed by a cartridge wall having an inner side, said cartridge comprising an electrically conducting material in the form of a solid piece of conducting material placed on the inner side of said cartridge wall, or in the form of one or more solid pieces or particles of conducting material incorporated in the wall of said cartridge; and

ii) placing the cartridge in an induction coil and sending alternating current through said coil to generate a magnetic field.

23. A method according to claim 22 including an automatic or semi-automatic assay of two or more specimens, comprising the additional steps of:

iii) placing each microscope slide individually in a cartridge including a chamber encompassed by a cartridge wall, said cartridge comprising an electrically conducting material in the form of a solid piece of conducting material placed on the inner side of said cartridge wall, or in the form of one or more solid pieces or particles of conducting material incorporated in the wall of said cartridge; and

iv) placing each cartridge individually in an induction coil and sending alternating current through said coil to generate a magnetic field.

24. A method for controlling the temperature of a biological specimen while testing or treating said biological specimen by employing a solid support member in combination with a carrier and an electromagnetic inductor, said inductor, comprising electrically conducting material on the surface facing the side of the carrier carrying the specimen, and generating a magnetic field with said electromagnetic inductor in the presence of the biological specimen.

25. The method for controlling the temperature of said biological specimen by employing solid support member in combination with an inductor according to claim 24, wherein said inductor comprises an induction coil and a power supply, said coil, preferably being sufficiently large to surround the support member, and said power supply capable of sending alternating current through said coil.

26. The method for controlling temperature according to claim 25, wherein said power supply includes an AC power supply.

27. A solid support member in combination with a carrier and an electromagnetic inductor for controlling the temperature of a specimen in direct or indirect contact with a solid support member by using induction heating, said support member being a cartridge for the carrier (e.g. a microscope slide) support member for testing or treating a specimen of biological material, and said support member comprising electrically conducting material on the surface facing the side of the carrier carrying the specimen and said electromagnetic inductor capable of generating an oscillating magnetic field to cause induction heating in the electrically conducting material of the support member.

28. A solid support member in combination with an inductor according to claim 27, wherein said inductor comprises an induction coil and a power supply, said coil being sufficiently large to surround the support member and said power supply being able to send alternating current through said coil.

29. A solid support member according to claim 27 wherein said power supply includes an AC power supply.

30. A solid support member according to claim 29 wherein said AC power supply includes a frequency in the range of between 1 Hz and 500 kHz.

31. A solid support member in combination with a carrier and an electromagnetic inductor for controlling the temperature of a specimen in direct or indirect contact with a solid support member providing induction heating, said support member being a cover plate for a microscope slide support member for testing or treating a specimen of biological material, and said support member comprising electrically conducting material on the surface facing the side of the carrier carrying the specimen and said electromagnetic inductor being able to generate an oscillating magnetic field, causing induction heating in the electrically conducting material of the support member.