OB fusion protein compositions and methods

• US 6,936,439 B2
• Filed: 10/06/2003
• Issued: 08/30/2005
• Est. Priority Date: 11/22/1995
• Status: Expired due to Fees

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Hecht, Randy Ira, Pelleymounter, Mary Ann, Toombs, Christopher Francis, Mann, Michael B.
• Primary Examiner(s): Chan, Christina
• Assistant Examiner(s): HUYNH, PHUONG N
• Application Number:
  US10/679,999
• Publication Number:
  US 20040067520A1
• Time in Patent Office: 694 Days
• Field of Search: 536/23.4, 435/69.1, 435/252.3, 435/320.1
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/2264 Obesity-gene products, e.g. leptin
• CPC Class Codes: C07K 14/5759 Products of obesity genes, e.g. leptin, obese (OB), tub, fat
• CPC Class Codes: C07K 19/00 Hybrid peptides , i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

The present invention relates to Fc-OB fusion protein compositions, methods of preparation of such compositions and uses thereof. In particular, the present invention relates to a genetic or chemical fusion protein comprising the Fc immunoglobulin region, derivative or analog fused to the N-terminal portion of the OB protein, derivative or analog.

Claims

1. An isolated DNA sequence comprising SEQ ID NOS:

7 or 8 encoding a human OB fusion protein comprising SEQ ID NO;

9;

or an isolated DNA sequence comprising SEQ ID NOS;

10 or 11 encoding a human OB fusion protein comprising SEQ ID NO;

12;

or an isolated DNA sequence comprising SEQ ID NOS;

13 or 14 encoding a human OB fusion protein comprising SEQ ID NO;

15;

or an isolated DNA sequence comprising SEQ ID NOS;

16 or 17 encoding a human OB fusion protein comprising SEQ ID NO;

18.

2. A process of producing a fusion protein wherein the process comprises expressing the DNA sequence of claim 1 in a transformed host cell and isolating the fusion protein.

3. A vector comprising the DNA sequence of claim 1.

4. An isolated host cell transformed or transfected with the DNA sequence of claim 1.

5. The host cell of claim 4, wherein the host cell produces the fusion protein for use in increasing insulin sensitivity.