Detection and typing of human papillomavirus using PNA probes

• US 6,936,443 B2
• Filed: 11/01/2002
• Issued: 08/30/2005
• Est. Priority Date: 04/03/2000
• Status: Expired due to Fees

• Current Assignee: Cytcy Corporation, Cytyc Corporation (Hologic Incorporated)
• Original Assignee: Cytyc Corporation (Hologic Incorporated)
• Inventors: Cohenford, Menashi A., Lentrichia, Brian
• Primary Examiner(s): Fredman, Jeffrey
• Assistant Examiner(s): STRZELECKA, TERESA E
• Application Number:
  US10/286,387
• Publication Number:
  US 20030143529A1
• Time in Patent Office: 1,033 Days
• Field of Search: 435/91.2, 536/23.1, 536/24.3
• US Class Current: 435/91.2
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: C12Q 1/708 for papilloma
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The invention provides materials and methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV. Novel primer sequences are also provided.

Claims

1. An essentially pure nucleic acid comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:

9, 10, 12, 13, 17-19 and 21.

2. The nucleic acid of claim 1, comprising SEQ ID NOS:

9.

3. The nucleic acid of claim 1, comprising SEQ ID NOS:

10.

4. The nucleic acid of claim 1, comprising SEQ ID NOS:

12.

5. The nucleic acid of claim 1, comprising SEQ ID NOS:

13.

6. The nucleic acid of claim 1, comprising SEQ ID NOS:

17.

7. The nucleic acid of claim 1, comprising SEQ ID NOS:

18.

8. The nucleic acid of claim 1, comprising SEQ ID NOS:

19.

9. The nucleic acid of claim 1, comprising SEQ ID NOS:

21.

10. A method for amplifying a target nucleic acid comprising selecting a primer selected from the group consisting of SEQ ID NOS:

9, 10, 12, 13 17-19 and 21, and amplifying said target nucleic acid.

11. The method of claim 10, wherein the method comprises a polymerase chain reaction.

12. The method of claim 10, further comprising selecting a primer selected from the group consisting of SEQ ID NOS:

8 and 16.

13. The method of claim 10, wherein a target nucleic acid of the amplification is a high risk strain of human papilloma virus.

14. The method of claim 10, further comprising selecting a probe comprising a nucleic acid analog to block full length amplification of a non-target nucleic acid.

15. The method of claim 13, wherein the non-target nucleic acid is of a low risk strain of human papilloma virus.