Detecting device for fluorescent-labeled material
First Claim
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1. A device for detecting visible fluorescence emitted from a fluorescent-labeled sample comprising:
- (a) a light source which produces incident light;
(b) a light guide to transmit light from the light source to an excitation filter or bandpass filter;
(c) a dichroic beamsplitter which reflects all incident light of a predetermined wavelength to a sample;
(d) a longpass filter or barrier filter through which light from the beamsplitter is transmitted to a line of sight.
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Abstract
A fluorochrome-marked sample is excited with the proper wattage and wavelength of light using an exciter or bandpass filter and beamsplitting mirror device. This causes the sample to emit light of a slightly higher wavelength than that of excitation. A subsequent barrier filter of the proper configuration placed between the sample and the observer allows this longer wavelength to be observed as fluorescence. This technique is particularly useful for viewing large biological specimens such as fish.
27 Citations
7 Claims
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1. A device for detecting visible fluorescence emitted from a fluorescent-labeled sample comprising:
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(a) a light source which produces incident light; (b) a light guide to transmit light from the light source to an excitation filter or bandpass filter; (c) a dichroic beamsplitter which reflects all incident light of a predetermined wavelength to a sample; (d) a longpass filter or barrier filter through which light from the beamsplitter is transmitted to a line of sight. - View Dependent Claims (2, 3, 4)
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5. A method of examining a live animal to detect fluorescence comprising:
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contacting said live animal with incident light which has been transmitted through a dichroic beamsplitter which reflects all incident light of a predetermined wavelength to the live animal containing a fluorochrome; transmitting light through a barrier filter or longpass filter located in eyeglasses; and observing light emitted from the live animal through the barrier filter or longpass filter. - View Dependent Claims (6, 7)
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Specification