Microfluidic systems in the electrochemical detection of target analytes
First Claim
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1. A microfluidic device for the detection of a target analyte in a fluid sample comprising:
- a) a solid support member;
b) a sample handling module including a sample handling well formed in said support member to receive and store said sample;
c) a sample inlet port to said microfluidic device;
d) a first microchannel formed in said support member coupled to and extending between said sample handling well and said sample inlet port;
e) a detection well formed in said support member and a detection electrode positioned in said detection well, said detection electrode being provided with a self-assembled monolayer; and
a binding ligand; and
, f) a second microchannel formed in said support member and extending between said sample handling well and said detection well for the flow of said fluid sample there between.
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Abstract
The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
384 Citations
14 Claims
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1. A microfluidic device for the detection of a target analyte in a fluid sample comprising:
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a) a solid support member;
b) a sample handling module including a sample handling well formed in said support member to receive and store said sample;
c) a sample inlet port to said microfluidic device;
d) a first microchannel formed in said support member coupled to and extending between said sample handling well and said sample inlet port;
e) a detection well formed in said support member and a detection electrode positioned in said detection well, said detection electrode being provided with a self-assembled monolayer; and
a binding ligand; and
,f) a second microchannel formed in said support member and extending between said sample handling well and said detection well for the flow of said fluid sample there between. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A microfluidic device for the detection of a target analyte in a fluid sample comprising:
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a) a solid support member;
b) a reaction module formed in said support member;
c) a sample inlet port to said microfluidic device;
d) a first microchannel formed in said support member coupled to and extending between said reaction module and said sample inlet port;
e) a detection well formed in said support member and a detection electrode positioned in said detection well, said detection electrode being provided with a self-assembled monolayer; and
a binding ligand; and
,f) a second microchannel formed in said support member and extending between said reaction module and said detection well for the flow of said fluid sample there between. - View Dependent Claims (14)
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Specification