Error detection in chemical array fabrication
First Claim
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1. A method of forming an addressable array of chemical moieties on a substrate, comprising:
- (a) providing a substrate having multiple feature locations and multiple test locations;
(b) depositing a reagent drop set from a single dispenser onto each feature location so as to attach a corresponding moiety for each feature location, wherein said deposition of a reagent drop set is a cycle;
(c) repeating step (b) at each feature location until the addressable array is formed;
wherein for each feature location of said addressable array a multi-dispenser drop group is deposited thereon, wherein said multi-dispenser drop group is formed over two or more cycles of (b) and (c); and
wherein said drops deposited in said two or more cycles are deposited from at least two or more different dispensers;
the method additionally comprising;
(d) depositing individual reagent drops of a previously formed multi-dispenser drop group from said different dispensers at respective, separate test locations on the substrate;
wherein each of said separate test locations does not include a previously deposited drop; and
(e) detecting said individual reagent drops at said respective test locations.
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Abstract
A method, apparatus, and computer program product, for forming an addressable array of chemical moieties on a substrate. The method may include, for each of multiple locations on the substrate, depositing a reagent drop set during a cycle so as to attach a corresponding moiety for that location. This may be repeated as required, until the addressable array is formed. Test location are also formed by depositing drops from dispensers in a manner which can facilitate detection of dispenser errors.
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Citations
39 Claims
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1. A method of forming an addressable array of chemical moieties on a substrate, comprising:
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(a) providing a substrate having multiple feature locations and multiple test locations;
(b) depositing a reagent drop set from a single dispenser onto each feature location so as to attach a corresponding moiety for each feature location, wherein said deposition of a reagent drop set is a cycle;
(c) repeating step (b) at each feature location until the addressable array is formed;
wherein for each feature location of said addressable array a multi-dispenser drop group is deposited thereon, wherein said multi-dispenser drop group is formed over two or more cycles of (b) and (c); and
wherein said drops deposited in said two or more cycles are deposited from at least two or more different dispensers;
the method additionally comprising;
(d) depositing individual reagent drops of a previously formed multi-dispenser drop group from said different dispensers at respective, separate test locations on the substrate;
wherein each of said separate test locations does not include a previously deposited drop; and
(e) detecting said individual reagent drops at said respective test locations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of forming an addressable array of chemical moieties on a substrate, comprising:
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(a) providing a substrate having multiple feature locations and multiple test locations, where said multiple test locations are present in a test pattern area separate from the multiple feature locations;
(b) depositing a reagent drop set from a single dispenser onto each feature location so as to attach a corresponding moiety for each feature location, wherein said deposition of a reagent drop set is a cycle;
(c) repeating step (b) at each feature location until the addressable array is formed;
wherein for each feature location of said addressable array a multi-dispenser drop group is deposited thereon, wherein said multi-dispenser drop group is formed over two or more cycles of (b) and (c); and
wherein said drops deposited in said two or more cycles are deposited from at least two or more different dispensers;
the method additionally comprising;
(d) depositing individual reagent drops of a previously formed multi-dispenser drop group from said different dispensers at respective, separate test locations on the substrate;
wherein each of said separate test locations does not include a previously deposited drop; and
(e) detecting said individual reagent drops at said respective test locations. - View Dependent Claims (21, 22, 23, 24)
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25. A method of forming an addressable array of polymers on a substrate, comprising:
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(a) providing a substrate having multiple feature locations and multiple test locations;
(b) depositing a reagent drop set from a single dispenser onto each feature location so as to attach a monomeric unit of the corresponding polymer for each feature location, wherein said deposition of a reagent drop set is a cycle;
(c) repeating step (b) at each feature location until the addressable array is formed;
wherein for each feature location of said addressable array a multi-dispenser drop group is deposited thereon, wherein said multi-dispenser drop group is formed over two or more cycles of (b) and (c); and
wherein said drops deposited in said two or more cycles are deposited from at least two or more different dispensers;
the method additionally comprising;
(d) depositing individual reagent drops of a previously formed multi-dispenser drop group from said different dispensers at respective, separate test locations on the substrate;
wherein each of said separate test locations does not include a previously deposited drop; and
(e) detecting said individual reagent drops at said respective test locations. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method of forming multiple addressable arrays of chemical moieties on a substrate, comprising for each array:
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(a) providing a substrate having multiple feature locations and multiple test locations;
(b) depositing a reagent drop set from a single dispenser onto each feature location so as to attach a monomeric unit of the corresponding polymer for each feature location, wherein said deposition of a reagent drop set is a cycle;
(c) repeating step (b) at each feature location until the addressable array is formed;
wherein for each feature location of said addressable array a multi-dispenser drop group is deposited thereon, wherein said multi-dispenser drop group is formed over two or more cycles of (b) and (c); and
wherein said drops deposited in said two or more cycles are deposited from at least two or more different dispensers;
the method additionally comprising;
(d) depositing individual reagent drops of a previously formed multi-dispenser drop group from said different dispensers at respective, separate test locations on the substrate;
wherein each of said separate test locations does not include a previously deposited drop and wherein the drops are deposited at a separate test pattern area between arrays with the number of test locations of the test pattern area being less than one tenth the number of feature locations in the smallest of the arrays which the test pattern area is between; and
(e) detecting said individual reagent drops at said respective test locations. - View Dependent Claims (39)
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Specification