Arrays with elongated features

• US 6,946,285 B2
• Filed: 04/29/2002
• Issued: 09/20/2005
• Est. Priority Date: 04/29/2002
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Bass, Jay K.
• Primary Examiner(s): GOLDBERG, JEANINE ANNE
• Application Number:
  US10/135,944
• Publication Number:
  US 20030203368A1
• Time in Patent Office: 1,240 Days
• Field of Search: 435/287.2, 435/91.1, 435/6, 435/5, 435/7.1, 435/91.2, 536/23.1
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00554 Physical means
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B29C 31/045 moving along predetermined circuits or distributing the material according to predetermined patterns
• CPC Class Codes: B29C 41/20 incorporating preformed parts or layers, e.g. moulding inserts or for coating articles
• CPC Class Codes: B29C 41/36 Feeding the material on to the mould, core or other substrate
• CPC Class Codes: B41J 2/2114 Ejecting transparent or white coloured liquids, e.g. processing liquids B41J2/211 takes precedence
• CPC Class Codes: B41J 3/407 for marking on special material
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

Arrays having multiple biopolymer features on a substrate, including elongated features which have a length/width ratio greater than 1.3 or more. Such arrays may be oriented in various directions and of shapes described, and fabricated using apparatus, methods, or computer program products of the invention.

Claims

1. An addressable array having multiple biopolymer features on a substrate, wherein a majority of the features are elongated features which have a length/width ratio that is greater than 1.3 and have a narrower portion of the width which is intermediate wider portions, wherein each feature of said array comprises multiple copies of a biopolymer and wherein the lengths of at least a majority of the elongated features are orientated in a common direction, and at least some of the elongated features are oriented in different directions.

2. A biopolymer array according to claim 1 wherein the length/width ratio is greater than 1.5.

3. A biopolymer array according to claim 1 wherein the length/width ratio is greater than 3.

4. A biopolymer array according to claim 1 wherein the elongated features carry polynucleotides of different sequence.

5. A biopolymer array according to claim 1 having at least 100 of the elongated features with a length of less than 1 cm.

6. A biopolymer array according to claim 1 having at least 5000 of the elongated features with a length less than 1 mm.

7. A biopolymer array according to claim 6 having at least 5000 of the elongated features with a length less than 200 micrometers.

8. An addressable array having multiple biopolymer features on a substrate, wherein at least a majority of the features of the array are elongated features which have a length/width ratio greater than 1.3 and have a narrower portion of the width which is intermediate wider portions, and the lengths of said majority are oriented in a common direction, and wherein each feature of said array comprises multiple copies of a biopolymer and wherein the lengths of at least some of the elongated features are oriented in different directions.

9. The array of claim 8 wherein at least 60% of the features of the array are the elongated features.

10. The array of claim 8 wherein at least 70% of the features of the array are the elongated features.

11. The array of claim 8 wherein at least 80% of the features of the array are the elongated features.

12. The array of claim 8 wherein at least 90% of the features of the array are the elongated features.

13. The array of claim 8 wherein the elongated features carry polynucleotides of different sequence.

14. The array of claim 8, wherein the array includes at least 100 elongated features.

15. The array of claim 14 wherein the at least 100 elongated features have a length less than 1 cm.

16. The array of claim 8 wherein the array includes at feast 5000 elongated features.

17. The array of claim 16 wherein the at least 5000 elongated features have a length less than 1 mm.

18. The array of claim 17 wherein the at least 5000 elongated features have a length less than 200 micrometers.

19. An array having multiple biopolymer features on a substrate, including at least 100 elongated features have a length/width ratio greater than 1.3 and have a narrower portion of the width which is intermediate wider portions, and wherein each feature of said array comprises multiple copies of a biopolymer.

20. The array of claim 19 wherein the at least 100 elongated features have a length of less than 1 cm.

21. The array at claim 19 wherein the at least 100 elongated features have a length of less than 1 mm.

22. The array of claim 21 wherein the at least 100 elongated features have a length of less than 200 micrometers.

23. The array of claim 19 wherein the at least 100 elongated features carry polynucleotides of different sequence.

24. The array of claim 19 wherein at least 60% of the features of the array are the elongated features.

30. The array of claim 24 wherein the majority of the features of the array are the elongated features.

25. An array having multiple biopolymer features on a substrate, including at least 5000 elongated features which have a length/width ratio greater than 1.3 and have a narrower portion, which is intermediate wider portions.

26. The array of claim 25 wherein the at least 5000 elongated features have a length of less than 1 cm.

27. The array of claim 25 wherein the at least 5000 elongated features have a length of less than 1 mm.

28. The array of claim 27 wherein the at least 5000 elongated features have a length of less than 200 micrometers.

29. The array of claim 25 wherein the at least 5000 elongated features carry polynucleotides of different sequence.