Progenitor cells, methods and uses related thereto
First Claim
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1. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
- obtaining a cell suspension from an animal tissue selected from pancreatic tissue or pancreatic ductal tissue, wherein said cell suspension comprises at least one progenitor cell;
treating the cell suspension with a preparation comprising epidermal growth factor, a cAMP elevating agent, and a steroid or corticosteroid; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population that is at least about 75% pure.
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Abstract
The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.
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Citations
12 Claims
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1. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
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obtaining a cell suspension from an animal tissue selected from pancreatic tissue or pancreatic ductal tissue, wherein said cell suspension comprises at least one progenitor cell;
treating the cell suspension with a preparation comprising epidermal growth factor, a cAMP elevating agent, and a steroid or corticosteroid; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population that is at least about 75% pure. - View Dependent Claims (2, 3, 9, 10, 11, 12)
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4. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
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obtaining a cell suspension from an animal tissue selected from pancreatic tissue or pancreatic ductal tissue, wherein said cell suspension comprises at least one progenitor cell;
treating the cell suspension with a preparation comprising epidermal growth factor, a cAMP elevating agent, and a steroid or corticosteroid; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population that is at least about 80% pure. - View Dependent Claims (5, 6, 7)
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8. A method for preparing a substantially pure non-adherent population of progenitor cells comprising:
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providing an animal tissue selected from pancreatic tissue or pancreatic ductal tissue;
disrupting said animal tissue so as to obtain a cell suspension comprising at least one progenitor cell;
treating the cell suspension with a preparation comprising a fibroblast growth factor and an epidermal growth factor; and
allowing proliferation of said at least one progenitor cell such that a substantially pure non-adherent progenitor cell population is obtained, thereby obtaining a substantially pure non-adherent progenitor cell population at least about 75% pure.
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Specification