Methods and kits for obtaining and analyzing skin samples for the detection of nucleic acids
First Claim
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1. A method for quantitating relative expression of a ribonucleic acid (RNA) from a skin sample, comprising:
- (a) applying an adhesive tape to the skin, under conditions allowing for isolation of a skin sample adhering to the adhesive tape;
(b) detecting the RNA from the skin sample; and
(c) comparing the level of the RNA in the skin sample to a control sample, thereby quantitating relative expression of the RNA.
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Abstract
Disclosed is a method for removing polynucleotide from the skin. This polynucleotide can be used to detect dermatitis and distinguish an irritant reaction from an allergic reaction by characterizing the polynucleotide according to the polypeptide which it encodes. Additionally, provided are methods for non-invasive isolation of samples from the skin as well as kits for use in the methods provided herein.
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Citations
13 Claims
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1. A method for quantitating relative expression of a ribonucleic acid (RNA) from a skin sample, comprising:
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(a) applying an adhesive tape to the skin, under conditions allowing for isolation of a skin sample adhering to the adhesive tape;
(b) detecting the RNA from the skin sample; and
(c) comparing the level of the RNA in the skin sample to a control sample, thereby quantitating relative expression of the RNA. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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2. The method of claim 2, wherein the skin sample comprises stratum corneum cells and cells associated with the stratum corneum which are removed by application and removal of the adhesive tape.
Specification